Methods: Twenty-four genotype (GT) 1 and twenty GT 2/3 HCV-infected subjects were randomized into 4 treatment groups: placebo or IDX21437 at 50, 150 or 300 mg QD x 7 days. HCV RNA was quantified using COBAS® AmpliPrep/TaqMan®v2.0, LLQ<25 IU/mL. Genotyping was performed with Versant HCV Genotype (LiPA) 2.0. Plasma concentrations of IDX21437 and its nucleoside metabolite, IDX20664, were quantified with a validated LC/MS/MS. Results: Thirty-nine GT 1-3 subjects received IDX21437. Following preliminary analyses of phar-macokinetic and pharmacodynamic

results, the protocol Palbociclib price was amended to discontinue enrollment into the 50 mg and 150 mg treatment groups. IDX21437 was safe and well tolerated. There were no serious adverse events, discontinuation due to adverse events (AEs), patterns of AEs or laboratory abnormalities related to IDX21437. Plasma exposures of IDX21437 and its nucleoside metabolite, IDX20664, increased with dose and were comparable to HS. IDX20664 exhibited a plasma half-life of 20-30 h. The mean maximum viral load (log10 IU/ mL) reductions from baseline are presented below. There were no viral breakthroughs. Conclusions: IDX21437 demonstrated potent, pan-genotypic activity in HCV-infected subjects at 300 mg for 7 days. To date, IDX21437 has been well tolerated

with no safety signal observed in either HS or HCV-infected subjects. These data support testing of 300 mg QD in a planned phase II clinical trial to evaluate the combination of IDX21437 and samatasvir, buy Daporinad a pan-genotypic NS5A inhibitor. Overall, the antiviral activity, PK and safety of IDX21437 in GT 1, 2 and 3 HCV-infected subjects dosed QD x 7 days support further development of IDX21437 as the backbone of future all-oral, pan-genotypic antiviral regimens. Mean maximum viral load (log10 IU/mL) reductions from selleck chemical baseline Disclosures: Edward J. Gane – Advisory Committees or Review Panels: Novira, AbbVie, Novartis, Gilead Sciences, Janssen Cilag, Vertex, Achillion, Tekmira, Merck, Ide-nix; Speaking and Teaching: AbbVie, Novartis, Gilead

Sciences, Janssen Cilag Xiao-Jian Zhou – Employment: Idenix Pharmaceuticals Marie-Francoise Temam – Employment: Idenix Pharmaceuticals Inc. Jie Chen – Employment: Idenix Pharmaceuticals Dodie Frank – Employment: Idenix Pharmaceuticals Eileen F. Donovan – Employment: Idenix Pharmaceuticals Keith Pietropaolo – Employment: Idenix Pharmaceuticals, Inc. Douglas L. Mayers – Management Position: Idenix Pharmaceuticals The following people have nothing to disclose: Eric Sicard, Serghei Popa Purpose/Background: Since they display a high genetic barrier to drug resistance and are pan-genotypic, nucleoside HCV inhibitors are the preferred candidates in the pursuit to achieve 100% sustained virological response (SVR) with one molecule.