Since April 2005, the M. D. Anderson CB Bank has banked for clinical use over 10 000 CBUs obtained from four hospitals in Houston, Texas. According to the 2000 US Census, the racial make-up of Houston consists of 49.27% White, 25.31% Black or African American, 0.44% Native American, 5.31% Asian, 0.06% Pacific Islander, 16.46% other races, and Y-27632 nmr 3.15% two or more races. Hispanics or Latinos of any race constitute 37% of the population. Between July 2009 and February 2010, we surveyed the CCR5 genotypes of CBUs donated to the Bank under an M. D. Anderson Cancer Center-approved IRB protocol. These included 1538 CBUs collected from the Ben Taub General Hospital (BTGH) (n=668), The Woman’s Hospital of Texas (TWHT) (n=649), The Methodist
Hospital (TMH) (n=61), and the Saint Joseph Medical Center (SJMC) (n=160). CBUs derived from parents who were of western European, northern R428 cost European, eastern European, North American, or White South or Central American origin were grouped as Caucasian. Africans, African Americans, Black
South or Central Americans, Black Caribbeans, and people from the north coast of Africa were classified as Black. The Asian subgroup included Japanese, Korean, Chinese, Vietnamese, South Asian, Filipino and South East Asian. Middle Eastern, Samoan, Hawaiian, and other unspecified races were grouped as ‘others’. CBUs were also classified as of Hispanic or non-Hispanic origin independently of race. The ethnicities of the parents who donated the CBUs deposited in the M. D. Anderson CB Bank are summarized in Table 1. At the BTGH, 94.29% of the parents were Caucasian (93.67% considered themselves of Hispanic origin), 4.01% were Black, 1.70% were Asian, and 0.00% were in the ‘others’ category. At TWHT, 76.12% were Caucasian (22.37% of Hispanic origin), 14.25% were Black, 5.01% were Asian, and 4.62% were in the ‘others’ category. At TMH, 77.19% were Caucasian (27.19% of Hispanic origin), 20.18% were Black,
2.63% were Asian, and 0.00% were in the ‘others’ category. At the SJMC, 85.45% were Caucasian (79.55% of Hispanic selleck screening library origin), 11.82% were Black, 1.37% were Asian, and 1.36% were in the ‘others’ category. We screened CBUs for the CCR5Δ32 allele. DNA was extracted from a discarded portion of each CBU and the genomic region flanking the 32-bp deletion of the CCR5Δ32 allele was amplified using the polymerase chain reaction (PCR). The wild-type CCR5 allele generated a 196-bp product whereas the CCR5Δ32 allele generated a 164-bp product, i.e. 32 bp shorter than the wild type (Fig. 1). The PCR assay identified 134 CCR5Δ32/+CBUs (8.71%) and 10 CCR5Δ32/Δ32 CBUs (0.65%) among the 1538 CBUs genotyped. DNA sequencing of the PCR products from the CCR5Δ32/Δ32 CBUs demonstrated that 100% had theΔ32 allele (Fig. 2). The overall frequency of the CCR5Δ32 allele in the CBUs collected from these four hospitals was 10% (Table 2). However, the frequency of the CCR5Δ32 allele in the CBUs collected from the four hospitals varied significantly.