To investigate Selleck NVP-BSK805 the association between induction fold and cancer grade, one-way ANOVA test for linear trend was performed between mean induction fold and subdivided cancer grades (Figure 5D). For Prx I, slope = 0.6217, P =.02; for Trx1, slope = 0.4497, P =.02. For both cases, linear trends were considered statistically significant if P <.05. Clinicopathological information for each patient was provided by the supplier. Abbreviations: ANOVA, analysis of variance; Prx I, peroxiredoxin

I; qRT-PCR, quantitative real-time polymerase chain reaction; Trx1, thioredoxin 1. To examine the relationship between mRNA expression of Prx I and Trx1 and progress of cancer, we displayed the data as box-and-whisker plots (cancer phase versus induction fold mRNA expression) (Prx I, Figure 5B; Trx1, Figure 5C). In both Prx I and Trx1, there was a significant relationship www.selleckchem.com/products/fg-4592.html between the induction fold and increasing cancer phase, especially for metastatic cancer (comparison of Prx I expression from stage I to stage IV, P =.040; Trx1, P =.009). Stage IV (n = 12) was classified as metastatic cancer. In addition, we divided the cancer phases into subdivisions (stages I, IIA, IIB, IIIA, IIIB, IIIC, and IV) and compared these by induction fold expression. As shown in Figure 5D,

induction fold was associated with subdivisions of cancer stages (P =.0181 for Prx I and P =.0191 for Trx1) Correlation Between Prx I and Trx1 in Human Breast Cancer To investigate an association between Prx I and Trx1 in human breast cancer, we Vorinostat cost plotted the both induction folds in breast cancer as x-y plot (x-axis for that of Prx I mRNA; y-axis for that of Trx1 mRNA). Figure 6 depicts the correlation between induction folds of Prx I and Trx1 genes in breast cancer (Pearson

r = 0.6875; P <.0001), indicating an association between Prx I and Trx1 in breast cancer. Figure PRKACG 6 Correlation Between Peroxiredoxin I and Thioredoxin1 mRNA Expressions in Breast Cancer. Data of induction folds of Prx I and Trx1 in breast cancer shown in Figure 5A are displayed as a scatter plot. Details are in the legend of Figure 5. Abbreviations: Prx I, peroxiredoxin I; Trx1, thioredoxin 1. Preferential Overexpression of Prx I and Trx1 Protein in Human Breast Cancer Tissue To examine the expression of Prx I and Trx1 proteins, Western blot analysis was conducted of protein lysates from seven cancer tissue types (brain, breast, colon, kidney, liver, lung, and ovary) separated by SDS-PAGE. Both Prx I and Trx1 proteins appeared to be elevated at the highest level when compared with those of other tissues (Figure 7A). Western blot analysis of the human breast cancer samples revealed a band at approximately 40 kDa. Western blot analysis in Figure 7B showed that the band in the reducing gel was entirely shifted to several higher molecular weight forms as shown in the nonreducing gel, suggesting that the 40-kDa band represents the dimer form of Prx I.