Recently, a commercial complement kit containing standardized ELISA-based assays for the assessment of all three complement pathways in clinical laboratories has been released. In this kit the MBL LP pathway is measured in wells coated with mannan and the contribution from the CP is inhibited by the use of a blocking anti C1q antibody [20]. Contribution from the AP is avoided by a minimal dilution of sera 1:101. These assays were validated in three different laboratories and they demonstrated high stability and reproducibility. However, one major concern Metabolism inhibitor associated

with these assays is the interference of the AP when assessing the functional capacity of the LP. Using novel ELISA set-ups in the present study, the normal functional activity of the three complement activation pathways was determined using serum samples from 150 healthy Danish blood donors. The functional capacity of the CP, determined as deposition of C3 on immune complexes, showed a normal distribution with a mean activity of

101% (57·4–161·9%). The capacity for the AP was determined as the deposition of C3 on an LPS-coated surface and showed a range of to 54·8–129·2%, with a mean value of 91%. Because of Pexidartinib manufacturer the normal distribution of the AP and the CP functional pathway activity, the lower cut-off value of normal activity was defined as the mean –1·96 × SD, resulting in a lower cut-off value of normal complement activity for the AP at 63·5% and 61% for CP. As expected, and in agreement with Garred et al. [8] and Seelen et al. [21], the complement activation capacity for the MBL pathway among healthy blood donors showed a large variation range with a bimodal distribution. This is due mainly to the variations in degrees of oligomerization of MBL as the concentration of functional MBL is the primary limiting factor for the LP activity. This was confirmed by a strong positive correlation between the MBL serum Fludarabine mw concentration

and the functional MBL pathway activity (r2 = 0·70, P < 0·0001). Given the relatively high frequency of individuals with MBL deficiency in the general population, it is important to define a normal MBL activity range. In attempt to define a pathway activity, it was decided to define the meaningful cut-off value for normal MBL cut-off activity level as the lowest activity level measured in an XA/O individual (selected from genotyping of individuals with MBL pathway activities between 0 and 43%). The highest MBL pathway activity level measured in a XA/O individual among the genotyped donors was 8% (Table 1), while all O/O individuals among the genotyped donors had no functional MBL pathway activity.