a L. kirschneri isolate that matched with the reference strain of L. interrogans as well as with L. kirschneri at first place. Detection of differentiating peaks within the pathogenic genomospecies

This analysis was performed to attempt the identification of discriminating peaks for serovars used in this study. For this, both datasets of the two institutions were analyzed by using the statistical software ClinProTools (Bruker Daltonik GmbH, Bremen, Germany). Datasets of the genomospecies L. interrogans, L. kirschneri and L. borgpetersenii were screened for analogies and differences in their protein profile peak patterns in order to identify www.selleckchem.com/products/blz945.html specific peaks that would allow the discrimination of the analyzed serovars. As L. interrogans and L. kirschneri showed a very close relationship at the species level, these two genomospecies were analyzed independently from the species L. borgpetersenii. The individual strains were analyzed applying different algorithms of the software. For this,

the software selects peak combinations, which are most relevant for the separation of the analyzed dataset. Within the species L. interrogans individual protein peak sets were present for the serovar Pomona (3,206 Da, 3,220 Da and 3,234 Da) and the serovar Copenhageni (3,636 and 3,657 Da), resulting in visually unique peak patterns. In addition, individual peak patterns were present for the serovars Australis, and Icterohaemorrhagiae. Beyond that, it was possible to discriminate L. kirschneri serovar Grippotyphosa from L. interrogans strains with an individual protein peak at 8,097 Da (see Table 4). To ascertain whether strains PARP inhibition within the L. kirschneri species display different peak patterns, the protein spectra (MSP) of one field isolate of the serovar Pomona (LGL 511, see Table 2) was added to the dataset. aminophylline Comparison of the two L. kirschneri serovars showed a mass deviation from 8,097 Da to 8,081 Da for the L. kirschneri Pomona field isolate (data not shown). Discriminating peaks occurred also within the species L. borgpetersenii (Table 5). The serovars Saxkoebing and Tarassovi were separated by individual protein peaks

at 7,547 Da and 5,765 Da and showed a unique protein pattern each (see Table 5). Table 4 Differentiating peaks based on the statistical analysis of ClinProTools within the species L. interrogans and L. kirschneri genomospecies peak mass (m/z) representing the protein size in Dalton 3,206 3,220 3,234 3,636 3,657 5,526 6,191 6,327 7,358 8,097 L. interrogans Hebdomadis – - – - – + + – + – L. interrogans Australis – - – - – - + – + – L. interrogans Autumnalis – - – - – + + – + – L. interrogans Bratislava – - – - – + + – + – L. interrogans Canicola – - – - – + + – + – L. interrogans Copenhageni – - – ++ ++ + + + – - L. interrogans Hardjo – - – - – + + – + – L. interrogans Pomona ++ ++ ++ – - + + + – - L. interrogans Pyrogenes – - – - – + + – + – L.