21 Screening will result in identification of individuals who have an increased risk of kidney and cardiovascular morbidity and mortality. In people with type 2 diabetes and microalbuminuria, a reduction in AER has been documented with improved glycaemic control, blood pressure control,

lipid profile optimization and specific renoprotective therapy with ACEi, or ARB.1 Thus screening should not be reserved for known high risk Ferrostatin-1 chemical structure populations (e.g. age >40 years, Australian Aborigines, positive family history of kidney disease) but should be offered to all people with type 2 diabetes. The methods which can be used to assess urinary albumin and protein excretion include: Dipstick, Timed urine collection, either 24 h or overnight (usually 8 h) is considered the gold standard for the measurement check details of albuminuria.22 Shorter timed collection periods can be used (e.g. 4 h) but these are time consuming for both patients and staff. AER and ACR on early morning urine are preferred as these tests are not subject to concentration bias. Considerations in choosing a particular test for assessment of albuminuria include: The purpose for which the test is being performed, The evidence for how kidney function should be assessed consists mainly of

cross sectional studies assessing various diagnostic tests against a reference method. In various clinical situations, ACR has been proposed as both a screening and diagnostic test for kidney disease.23 However, many have recommended the use of ACR only in screening,24–27 as the test has a high false positive rate and low specificity. Albumin-to-creatinine ratio is also considered to have a useful monitoring role in diabetes with respect to detecting kidney disease progression and the evaluation of treatment effects.28 All of the original assessments of microalbuminuria were based on AER measurements in timed urine collections. AER measurements performed in this way are Histone demethylase still regarded as the gold standard for assessment of microalbuminuria. This presumes that the assay

technique is sufficiently sensitive, the inter-assay coefficient of variation is less than 15% and at least 2 of 3 urine samples are in the appropriate range before a diagnosis of microalbuminuria is made.29 Albuminuria is commonly measured in the clinical laboratory by one of the following methods: radioimmunoassay (RIA), nephelometry (NEPH), immunoturbidimetry (IT) or radial immunodiffusion (RID). All of these methods are available as commercial kits. RIA is considered as the reference method for albumin measurement as it is the longest established assay. In an evaluation of RID, IT, NEPH against RIA the intra and inter-assay coefficient of variation (CV) of the methods were not found to be significantly different.30 A second study has also found similar degrees of precision and accuracy between the RIA, RID, and IT methods.