6 Percentageinhibition(%)=Control−TreatedControl×100 Group-1: Vehicle control received 1% CMC (dose: 10 ml/kg). On the 8th day animals were sacrificed and the cotton pellets were removed surgically, freed from extraneous tissue then the weight of wet cotton pellets weights were noted, thereafter the wet cotton pellets were dried in oven for 24 h at 60 °C. After drying the cotton pellets were weighed again to get the weight of dry cotton pellets. Animals were weighed by using animal weighing balance initially before experimentation and at the end of study. All the data

was expressed as Mean ± S.E.M. Statistical significance between more than two groups was tested using one-way ANOVA Lapatinib followed by the Tukey test using computer based fitting program (Prism graph pad.). Statistical significance was taken as p < 0.05. The effect of methanolic leaf extract of A. vulgaris was studied at the doses of

200 mg/kg & 400 mg/kg per body weight. The results revealed that the methanolic extract of A. vulgaris shows dose dependant inhibition of weight of both wet and dry cotton pellets, The mean number of decrease in weight of both wet and dry cotton pellets for rats, which received 200 mg/kg & 400 mg/kg body weight of the extract was significant check details (p < 0.05) lower than those in the control rats. The extract was found to be most effective at a dose of 400 mg/kg body weight. The extract at the dose of 400 mg/kg had shown 55.3%

inhibition in weight these of wet cotton pellets and 64.06% inhibition in weight of dry cotton pellets, while the extract at the dose of 200 mg/kg had shown 33% inhibition in weight of wet cotton pellets and 20.07% inhibition in weight of dry cotton pellets 50% inhibition of implants when compared to that of control group animals as shown in the following Table 1 and in Figs. 1 and 2. Fig. 3, Fig. 4 and Fig. 5 show the exposed cotton pellets at the end of the study. There was no significant weight variation observed in the body weights of the animals shown in Table 2, which reveals no toxic effect of the extract. In the present study, the anti-inflammatory activity of the methanolic leaf extract of A. vulgaris has been established using cotton pellet granuloma method. Cotton pellet granuloma model is an indication of the proliferative phases of inflammation. Inflammation involved proliferation of macrophages, neutrophils and fibroblast, which are basic sources of granuloma formation.7 The results revealed that the extract at the dose of 400 mg/kg had shown 55.3% inhibition in weight of wet cotton pellets and 64.06% inhibition in weight of dry cotton pellets, while the extract at the dose of 200 mg/kg had shown 33% inhibition in weight of wet cotton pellets and 20.07% inhibition in weight of dry cotton pellets when compared to that of control group animals as shown in the following Table 1 and Figs. 1 and 2.