Amalgam also requires placement of retentive features that demands excessive removal of tooth structure that further weakens the already weakened non vital tooth. The use of dental amalgam is declining worldwide Decitabine solubility because of legislative, safety and environmental issues. We are in the era of adhesive dentistry. Adhesive restorations bond directly to the tooth
structure and reinforce weakened tooth structure.1 Restoration of endodontically treated teeth with resin-based composite has increased due to development of better, more reliable bonding systems. Composite core buildup provides the high bond strength to tooth structure and increased resistance to fracture.2 Composite core material should have a good bond strength to the pulpal floor dentin so that it enhances retention and maximizes the seal.2 Opportunity for restoration of non-vital teeth with resin-based composite has increased due to the development of better and more reliable dentin bonding systems. Various bonding agents were being introduced into the market. Most recent developments have focused on simplification of multistep bonding processes using different approaches i.e.,
total etch, two-step self-etch and all-in-one system. The laboratory parameter most commonly used to measure the bonding effectiveness with dentin adhesives is micro shear bond strength. Hence, the objective of this study was to compare and evaluate the microshear bond strength of coronal and pulpal floor dentin using three-generation dentin bonding systems. Materials and Methods Materials used were as follows: (1) Composite resin: Clearfil APX (Kuraray) (2) Bonding agents: XP Bond (Dentsply) – 5th generation, Clearfil SE Bond (Kuraray) – 6th generation, G Bond (GC) – 7th generation, (3) Acid etchant: 37% Phosphoric acid (d-tech), and (4) Storage media- saline (Figure 1a). Thirty human mandibular molars extracted for periodontal reasons were collected for the study (Figure 1b) and the teeth were cleaned with ultrasonic scalers and stored in saline. The occlusal enamel was removed with high-speed diamond disc to expose a flat mid coronal dentin.
2 mm thick slabs of coronal dentin and pulpal floor dentin samples were prepared by sectioning at midpoint between floor of the pulp chamber and Drug_discovery root furcation. These prepared dentinal slabs were finished with wet silicon carbide sand paper under a stream of water to create an uniform smear layer. Samples were divided into two major groups depending upon the dentin location are Group I: 30 Samples of coronal dentin and Group II: 30 Samples of dentin at floor of the pulp chamber. Each group was further subdivided into three subgroups (Figure (Figure2a2a--f)f) of 10 samples each depending upon the bonding agent used (Subgroup a – XP Bond, Subgroup b – Clearfil SE Bond, Subgroup c – G Bond). Figure 1 (a and b) armamentarium, material and study samples.