cance. Finally, they were not expressed in mock control samples at all, although this observation was not reliable in the case of cv. Lynx samples collected at 72 hai due to the above mentioned restrictions. To analyse the observed congru ities in more detail and to test whether or not the ex pression in the susceptible cv. Lynx is just a temporary Alisertib clinical phenomenon, a selection of six genes representing dif ferent functional categories was forwarded to qPCR ana lysis using the above mentioned inoculation time courses of the cultivar pairs Dream vs. Lynx and Sumai 3 vs. Florence Aurore. The analysed genes associated with DON detoxification Inhibitors,Modulators,Libraries are TaUGT3 and a homologue of the barley UDP glucosyltransferase gene HvUGT13248. Genes that are supposed to be involved in the resistance to DON accumulation are TaPDR1 and TaMDR1.
As Inhibitors,Modulators,Libraries representatives of the functional cat egories defence related and general a further putative serine protease gene and a 12 oxophytodienoate reductase gene were included. The qPCR data for the winter wheat cultivars Dream vs. Lynx showed similar expression pat terns for all tested genes as did the microarray experi ments. Consequently, a temporary and higher induction peak was found for Lynx at 72 hai compared to Dream. On the other hand, the transcripts of all tested genes peaked at 96 hai in the cv. Dream samples, while Lynx revealed suppressed or consistent inductions. In addition, a 4 fold induction was already observed be fore 72 hai for most of the cv. Dream alleles and the expressions were showing a general and increasing trend towards the peak at 96 hai.
Such a max imum induction at 96 hai has likewise Inhibitors,Modulators,Libraries been observed for the DON resistance candidate gene PDR5 like in infected spikes of the Chinese landrace Wangshuibai which represents one of the most important genetic resources for FHB and DON re sistance. Like the analysed genes TaPDR1 and TaMDR1, PDR5 like is like a plasma membrane ABC transporter which co segregates with Inhibitors,Modulators,Libraries the DON resistance QTL Qfhs. ndus 6BS from cv. Wangshuibai. Anacetrapib In the cultivar pair Sumai 3 vs. Florence Aurore the Fusarium induced expression levels obtained for the UDP glucosyltransferase and ABC transporter genes were showing typical curve characteristics in cv. Sumai 3 samples, starting with a low level induction at 8 hai, followed by a consistent increase up to the peak at 32 or 48 hai and showing a continuous downtrend thereafter.
In contrast, considerable inductions for the susceptible cv. Florence Aurore did not appear until 96 to 120 hai. Interest ingly, both UGT genes show induction peaks at 32 hai in cv. Sumai 3 while selleck chemicals both ABC transporter genes peak at 48 hai. Deviating induction patterns were observed for the representatives of the functional categories defence related and general. For all tested genes no expressions were measured from samples col lected at 336 hai. In summary, the expression profiles of genes related to detoxification in both resistant cultivars were following an early beginning ste