Collectively, these studies provide static evidence of the protective role of VWF on FVIII, but newer dynamic experimental techniques are also available. A recent study

used surface plasmon resonance to characterize the interaction between human anti-FVIII IgG inhibitors and FVIII in concentrates from different sources [45]. Although measuring an antigen–antibody interaction by surface plasmon resonance is a complex process, it has a strong validation method. Using this technique, inhibitor antibodies are arranged in ordinate fashion on the surface of a chip which is then oriented against the flow of FVIII product. As product passes over the chip, light from underneath the chip reflects on an antigen–antibody complex and emits a signal which is measured in units of AG-014699 solubility dmso resonance. The FVIII products compared in this study were pdFVIII/VWF, full-length rFVIII (pre-incubated or not with purified VWF) and B domain deleted rFVIII. Concentration ranges of FVIII were 6 to 0.024 nm for plasma-derived concentrate and 9 to 0.03 nm for recombinant concentrates. Antibodies were sourced from a child with high inhibitor titres against FVIII. Association of FVIII with antibody was monitored for 3–5 min and disassociation of the antigen–antibody complex

was followed for 5, 20 and 240 min. Whereas no antigen–antibody reaction was observed with pdFVIII/VWF despite increasing concentrations of FVIII, there was a strong dose-dependent increase in the antigen–antibody selleck inhibitor reaction with full-length rFVIII (without VWF) and B domain deleted rFVIII (Fig. 9). Most interesting, however, was the result observed with rFVIII and plasma-derived VWF. Although binding signals with rFVIII + VWF were lower than those measured with uncomplexed rFVIII as indicated by the lower scale on the y axis, a definite dose-dependent antigen–antibody reaction was apparent (Fig. 9). It can be envisaged that, in the case of pdFVIII/VWF, all FVIII molecules are bound to VWF and VWF acts as MCE公司 a ‘shield’ for FVIII. In the case of rFVIII + VWF, the fraction of ‘free’ FVIII molecules unable to bind to VWF could interact with antibody to induce an immune reaction. Preincubation

of rFVIII with increasing concentrations of plasma-derived VWF (ranging from 1:0.001 to 1:1) reduced the antigen–antibody reaction to a low value in a dose-dependent manner. However, when results were displayed as the per cent reduction in binding signal relative to that with uncomplexed full-length rFVIII (as the reference value), a sigmoid curve was produced (Fig. 10). Intriguingly, the maximum relative reduction in binding signal to 20% of that of the reference value by addition of VWF to rFVIII corresponds closely with the fraction of FVIII unable to bind to VWF as discussed previously. Evidence is accumulating to suggest that differences in the reactivity of FVIII concentrates with inhibitor plasmas are influenced by their VWF content.