The specificity primers of JAK2617F gene mutation therefore the primers of CALR gene had been designed at the same time. The JAK2V617F and CALR gene primers were labeled with Cy5 fluorescence, all of the primers had been mixed in one single pipe for multiplex PCR together with PCR prodcuts had been analysised by capillary electrophoresis. Then detection restriction and susceptibility of MPCE had been evaluated, and compared to comercial diagnostic kit. JAK2V617F and CALR gene mutations could possibly be detect by MPCE in a single PCR test. JAK2V617F mutation could be recognized at 0.01 ng genomic DNA, two fold positive JAK2V617F and CLAR gene mutations could possibly be detected at 0.1 ng genomic DNA, at the least 0.1per cent JAK2V617F good mutation could be recognized. The persistence between MPCE and commercial diagnostic gene mutation kit ended up being 100%. Its developed that an innovative new gene mutation recognition way of JAK2 V617F and CLAR gene predicated on MPCE inside our experiment and it will be properly used as a unique reagent for molecular diagnosis of MPN patients.Its developed that a fresh gene mutation recognition way of JAK2 V617F and CLAR gene considering MPCE inside our experiment and it will be utilized as an innovative new reagent for molecular analysis of MPN customers. a prospective study ended up being carried out in 7 health facilities, 45 clients with MDS (≥ 60 yrs old) and MDS-transformed AML from October 2016 to January 2019 had been enrolled, using the median age 68.5 yrs . old. The danger stratification of customers was poor or very poor, according to IPSS-R score. The treament outcomes of decitabine coupled with CEG and decitabine combined with CAG were compared. The comparison of this two regiem showed that the DCEG regimen had benefits on total effective price (ORR, 86.4% vs 47.8%, correspondingly), overall survival time (OS) (10.0 months vs 6.0 months, correspondingly) and progression-free success time (PFS) (9.0 months vs 3.0 months, correspondingly). About 50% of MDS patients treated by DCEG regime reached PR or CR, with a median OS of 31 months. Multivariate analysis showed that patients with PR or CR after induction treatment and DCEG regimen had longer survival time (31months). The occurrence of bone tissue marrow suppression, infection and treatment-related mortality price were comparable amongst the two groups. Decitabine combined with CEG regimen could improve survival of clients with risky MDS and MDS-transformed AML. The final outcome associated with the reaserch has to be validated by a larger prospective randomized clinical trial.Decitabine combined with CEG program could increase the survival of patients with risky MDS and MDS-transformed AML. The final outcome associated with the reaserch needs to be validated by a bigger prospective randomized clinical trial. A complete of 39 hematopenia clients with suspected myeloid malignamies in division of Hematology associated with Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University from January 2018 to April 2019 had been addressed, 20 hot-spot genetics of myelodysplastic syndrome (MDS) had been detected. In connection with diagnostic type, there have been 7 instances of idiopathic cytopenia of undetermined importance (ICUS), 8 cases of clonal cytopenias of undetermined relevance (CCUS) and 24 situations of myeloid myeloid malignancies including 18 situations of MDS, 4 cases of myelodysplastic/myeloproliferative neoplasms (MDS/MPN) and 2 instances of acute myeloid leukemia. Positive Biohydrogenation intermediates mutation ended up being detected in 70.8% (17/24) of myeloid malignancy patients , and 72.7per cent (16/22) in MDS and MDS/MPN customers. The key mutation types had been ASXL1, TET2 and RUNX1. Weighed against gene unfavorable team, there were no sent. The medical data of 203 MDS patients who accepted Next Generation Sequencing (NGS) ended up being retrospectively analyzed in Nanfang Hospital, Southern healthcare University from December 2012 to October 2019. In accordance with whether or not the customers had U2AF1 gene mutation, the clients were SCH-527123 divided into U2AF1 mutated group and non-mutated team, in addition to relationship between gene mutation characteristics and medical manifestations and prognosis was reviewed. Then according to the huge difference of the mutation site of U2AF1, the customers in U2AF1 mutated group were divided into U2AF1 mutated group, therefore the correlation between gene mutation characteristics and prognosis was analyzed. The occurrence of U2AF1 mutation in MDS customers ended up being about 11.3% (23/203), while the mutation frequency of U2AF1 allele was Killer cell immunoglobulin-like receptor 32.5%. The male ratio in U2AF1 mutated ct the success time, AML transformation time, and response rate to hypomethylating agents in MDS customers. Besides, there aren’t any analytical differences in the medical qualities and prognosis of MDS customers between U2AF1 mutated team. Transplantation shows no considerable advantage for clients with U2AF1 mutation.The U2AF1 gene mutation dosage maybe not influence the success time, AML transformation time, and reaction price to hypomethylating agents in MDS patients. Besides, there are not any statistical differences in the medical attributes and prognosis of MDS clients between U2AF1S34 mutated group and U2AF1Q157/R156 mutated group. Transplantation shows no considerable benefit for clients with U2AF1 mutation. Medical data of 85 MM patients addressed with bontizomib from January 2015 to January 2019 were chosen and divided in to case group and control group accroding towards the occurred of herpes zoster. The clinical characteristic, treatment result and relevant element of herpes zoster were retrospective analyzed. The incidence of herpes zoster has lots of the multiple myeloma clients addressed with bortezomib. Lymphocytopenia took place before treatment, the effective use of cyclophosphamide, while the absence of prophylactic antiviral treatment are the crucial threat facets for herpes zoster, for which the physicians should attach great value.