immunization with all three IN genes elicited a large number of IN specific CD4 and CD8 T cells which simultaneously produced IFN h, IL 2 and TNF a. The quantity of CD4 and CD8 T cells double positive for IFN h, IL 2 and TNF an in mice receiving the IN genes was equally full of all three groups, and somewhat exceeded that in HDAC3 inhibitor the control vector immunized mice IN Gene Immunization Induces Specific Antibody Response Sera from BALB/c mice immunized with IN gene variants gathered after the completion of immunization was subjected to indirect ELISA on plates coated with the IN variants. IN gene immunization was found to produce IN specific IgG within the normal titers from 500 to 2500. IN a was equally reputable in every three teams, IgG titers varied from 200 to 3000. Immune system Interestingly, active opinion integrase was better recognized by the sera of mice immunized with divergent IN variant IN in e3: in this group the stop IN a titers reached 3000. Rats getting IN gene variant IN in e3 exhibited the anti IN clade B antibody titers. This compared with their high power to recognize the agreement effective integrase of FSU A strain. Titer of antibodies against IN of clade B in mice immunized with IN in e3 was lower than in mice receiving IN in gene. The overall antibody identification of IN clade T was poor with the normal antibody titers less than 1500. Reputation of mutant FSUA integrases IN IN and in in e3 was examined only in mouse groups immunized with individual versions. In vivo Assessment of the Effector Capacity of Antiintegrase Immune Response Next, we investigated whether the immunization with IN gene alternatives influences the in vivo expression of the transfected genes. For this, the expression was followed by us in the websites of immunization of the reporter gene encoding firefly luciferase co delivered as a 1:1 conjugating enzyme combination with IN gene variants. By day 21, the expression of luciferase in mice receiving Luc and IN genes had somewhat decreased, while little change was registered in mice receiving Luc gene as well as a clear vector. The decline in the luminescent signal emitted from the websites of injection of the reporter gene and the integrase was similar for IN a, IN IN and in in groups starting from day 9 and as much as day 21. Luminescence on day 21 inversely correlated with the finish point IFN c, IL 2 and dual IFNc/ IL 2 production by CD4 and with IFN c, TNF an and dual IFN c/TNF a production by CD8 T-cells. Similarly powerful inverse correlations were found involving the end point luminescence and the magnitude of integrase specific triple cytokine reaction of CD4 and of CD8 T cells. Apparently, as day 4, luminescence in the time points, directly correlated with the end point immune response.