Key Word(s): 1. nursing cooperation; 2. PEI ; 3. liver cancer; Presenting Author: JIAO LIU Additional Authors: WEI FEN XIE Corresponding Author: WEI FEN XIE Affiliations: Changzheng Hospital Objective: Tumor associated fibroblast (TAFs) can influence hepatocellular carcinoma (HCC) progression and metastasis. It was found that chemokines, such as CXCL-12, was activated in cancer cells and TAFs. This study aimed to investigated the interaction between cancer cells and TAFs and the effect of CXCL-12 in HCC microenvironment. Methods: Peri-tumor fibroblasts (PTFs) and TAFs were isolated from HCC patients. Quantitative PCR, western blotting,

immunofluorescence and immunohistochemistry were used to study the expression of CXCL-12 and its effect on signaling selleck inhibitor pathway. A coculture system was established to study the interaction in HCC microenvironment. Results: First, we demonstrated that TAFs presented more activated characteristics than PTFs. In coculture system, HCC cell line cocultured with TAFs exerted higher proliferation and migration abilities. In vivo, TAFs co-injected with HCC cells into nude mice subcutaneously showed larger tumor volumn. We also found that TAFs enhanced the Epithelial-Mesenchymal Transition

(EMT) in HCC cells , possibly by secreting CXCL-12. CXCL-12 induced activation of TGF-β /smad pathway, which displayed an important role in EMT and cell migration. CXCL-12 induced PI3K inhibitor the migration of HCC cells by binding to the receptor CXCR4; adding a CXCL-12 antibody to TAF-conditioned medium that inhibited the interaction between CXCL-12 and CXCR4 reduced migration. In addition, CXCL-12 secreted by HCC cells promoted transdifferentiation of PTFs to TAFs-like myofibroblast phenotype. Conclusion: Our data indicated that TAF accelerated HCC progression by CXCL12/TGF-β/EMT pathway,

and CXCL-12 promoted transdifferentiation of PTFs to TAFs-like myofibroblast phenotype. The findings suggested a potential clinical application. Key Word(s): 1. fibroblast; 2. HCC; Presenting Author: SHUQIN ZHANG Additional Authors: HAIYING SUN Corresponding Author: SHUQIN ZHANG Affiliations: Hepatology Hospital of Jilin Province Objective: Analysis of epidemiology 上海皓元 and the risk of death factors of primary liver cancer in our hospital in the past three years.2, Observation the changes of mortality and survival of primary liver cancer after multidisciplinary intervention since 2011. Methods: 721 cases of patients with primary liver cancer from January 2010 to January 2013, statistics include: gender, age, drinking history, family history, history of liver cirrhosis, HBV and HCV infection, HBVDNA and AFP level, the history of antiviral treatment. Results: 1,Men accounted for 83.91%, female accounted for 16.09%; aged 40-59 accounted for 67.