One hundred parameter initiation values ranging from 5 to 105 were tested and the best converging model with the smallest Sum Square of Error (SSE) was chosen for estimation of doubling time. Acknowledgements We thank Dr. C. Szekeres and Dr. R. Chen at USF Health core facilities for help with flow cytometry and statistical analyses, respectively. We thank B. White, B. Wisler and Y. Xi at the University of Notre Dame for their technical

assistance. This work was supported by grants from the National Institute of Allergy and Infectious Diseases to J.H.A. Electronic supplementary material Additional file 1:List of piggyBac insertion loci in the P. falciparum genome. Complete 17DMAG list ofpiggyBacinsertion loci identified thus far is provided along with the mutant name and insertion position relative to the coding sequences of the genome. (XLS 33 KB) Additional file 2:Best-fit growth curve models for doubling time estimation of mutant clones. The predicted best-fit and observed growth curves for each parasite clone is shown. (PDF 201 KB) Additional file 3:Lack of gene expression in mutant P. falciparum clones with insertions in the coding sequences. RT-PCR analysis confirms the knockout of gene

expression in mutant clones, selected for growth assays, with insertions in coding sequences. (PDF 157 KB) Selleckchem C188-9 References 1. Snow RW, Guerra CA, Noor AM, Myint HY, Hay SI:The global distribution of clinical episodes of Plasmodium falciparum malaria. Nature2005,434(7030):214–217.CrossRefPubMed 2. Yamey G:Roll Back Malaria: www.selleckchem.com/products/sch772984.html a failing global health campaign. Bmj2004,328(7448):1086–1087.CrossRefPubMed 3. Le Roch KG, Zhou Y, Blair PL, Grainger M, Moch JK, Haynes JD, De La Vega P, Holder Enzalutamide order AA, Batalov S, Carucci DJ,et al.:Discovery of gene function by expression profiling of the malaria parasite life cycle. Science2003,301(5639):1503–1508.CrossRefPubMed 4. Bozdech

Z, Llinas M, Pulliam BL, Wong ED, Zhu J, DeRisi JL:The Transcriptome of the Intraerythrocytic Developmental Cycle of Plasmodium falciparum.PLoS Biol2003,1(1):5.CrossRef 5. Florens L, Washburn MP, Raine JD, Anthony RM, Grainger M, Haynes JD, Moch JK, Muster N, Sacci JB, Tabb DL,et al.:A proteomic view of the Plasmodium falciparum life cycle. Nature2002,419(6906):520–526.CrossRefPubMed 6. Lasonder E, Ishihama Y, Andersen JS, Vermunt AM, Pain A, Sauerwein RW, Eling WM, Hall N, Waters AP, Stunnenberg HG,et al.:Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry. Nature2002,419(6906):537–542.CrossRefPubMed 7. LaCount DJ, Vignali M, Chettier R, Phansalkar A, Bell R, Hesselberth JR, Schoenfeld LW, Ota I, Sahasrabudhe S, Kurschner C,et al.:A protein interaction network of the malaria parasite Plasmodium falciparum.Nature2005,438(7064):103–107.CrossRefPubMed 8. Date SV, Stoeckert CJ Jr:Computational modeling of the Plasmodium falciparum interactome reveals protein function on a genome-wide scale. Genome Res2006,16(4):542–549.CrossRefPubMed 9.