Over recent years, the introduction of high-throughput genome sequencing and proteomic analyses [6] provided a source of exhaustive information about characterized bacterial isolates. Such data may now be included among the criteria used for taxonomic LB42708? identification. We recently proposed to use a polyphasic approach to describe new bacterial taxa that is based on their genome sequence, MALDI-TOF spectrum and main phenotypic characteristics [7-25]. Here we present a summary classification and a set of features for B. massiliosenegalensis sp. nov. strain JC6T together with the description of the complete genomic sequencing and annotation. These characteristics support the creation of the species B. massiliosenegalensis. The genus Bacillus (Cohn 1872) was created in 1872 [26] and currently consists of mainly Gram-positive, motile, and spore-forming bacilli.
Currently, 173 Bacillus species and 4 subspecies are validly published [27]. Members of the genus Bacillus are ubiquitous bacteria, mostly isolated from environmental sources. However, several species are associated with humans, either as pathogens or commensals [28]. Classification and features A stool sample was collected from a healthy 16-year-old male Senegalese volunteer patient living in Dielmo (rural village in the Guinean-Sudanian zone in Senegal), who was included in a research protocol. Written assent was obtained from this individual. No written consent was needed from his guardians for this study because he was older than 15 years old (in accordance with the previous project approved by the Ministry of Health of Senegal and the assembled village population and as published elsewhere [29].
) Both this study and the assent procedure were approved by the National Ethics Committee of Senegal (CNERS) and the Ethics Committee of the Institut F��d��ratif de Recherche IFR48, Faculty of Medicine, Marseille, France (agreement numbers 09-022 and 11-017). Several other new bacterial species were isolated from this specimen using various culture conditions, including the recently described Alistipes senegalensis, Alistipes timonensis, Anaerococcus senegalensis, Bacillus timonensis, Clostridium senegalense, Peptoniphilus timonensis and Paenibacillus senegalensis, Herbaspirillum massiliense, Kurthia massiliensis, Brevibacterium senegalense, Aeromicrobium massilense, Cellulomonas massiliensis, Senegalemassilia anaerobia, Peptoniphilus obesi, Peptoniphilus senegalensis, Enterobacter massiliensis, Alistipes obesi, Peptoniphilus grossensis, Brevibacillus massiliensis [7-25].
The fecal specimen was conserved at -80��C after collection. Strain JC6T was (Table 1) was isolated in January 2011 by cultivation on 5% sheep blood-enriched Brain Heart infusion (BHI) agar (Becton Dickinson, Heidelberg, Germany). The strain exhibited Dacomitinib a 97.3% nucleotide sequence similarity with B. siralis (Pettersson et al. 2000), the phylogenetically closest Bacillus species (Figure 1).