THC exposure resulted in a significant reduction in the T cell response to the local form of lysozyme after pretreatment of the macrophages with nanomolar drug concentrations. Hence, these, and other studies, implicate the CB2 as representing a constituent part of a circle of G protein coupled receptor indication transductional systems, inclusive of chemokine receptors, that act coordinately to regulate macrophage migration. It has been shown also that the CB2 is involved in cannabinoid mediated inhibition of processing of antigens by macrophages. In studies performed Lapatinib 388082-77-7 to examine the result of 9 THC on the processing of whole lysozyme by macrophages, it was shown that 9 THC reduced the ability of a macrophage hybridoma to operate being an antigen presenting cell based on its ability to secrete IL 2 upon stimulation of the soluble protein antigen distinct helper T cell hybridoma. But, 9 THC did not affect when the macrophages introduced a synthetic peptide of the antigen to T-cells, suggesting that the drug interfered with antigen processing IL 2 production, not peptide display. Whereas the relatively inactive stereoisomer CP56667 didn’t the cannabinoid inhibition of the T cell reaction to native lysozyme was stereoselective, consistent with the effort of a cannabinoid receptor for the reason that bioactive CP55940 decreased T cell activation. The macrophage Eumycetoma hybridoma expressed mRNA for CB2 although not for CB1. More over, the CB1 selective antagonist SR141716A didn’t reverse the suppression due to 9 THC while the CB2 selective antagonist SR144528 totally blocked the 9 THC suppression of the T cell response. Collectively, these results implicated macrophages as the goal of cannabinoid inhibition of antigen processing in a mode that has been connected functionally to CB2. CLINICAL IMPLICATIONS/APPLICATIONS Cannabinoids, as mapk inhibitor ligands that transmission through cannabinoid receptors, may be particularly of use as agents for therapeutic manipulation of hyperinflammatory immune reactions within the CNS. These substances are very lipophilic and within this context easily penetrate the BBB, a challenge that’s asked to a variety of agents that have therapeutic potential. More over, through the application of appropriately manufactured molecules, it may be possible to specifically target the CB2, an ailment that would obviate if the CB1 were stimulated also technology of annoying psychotropic consequences that could possibly be engendered. Microglia, as macrophage like cells, throughout activation also up manage an array of cell surface receptors which may be crucial in regeneration and/or destruction of the CNS.