The levels of MGP mRNA expression were quantified using

real-time find more PCR (RT-PCR), and the levels of protein expression were characterized by Western blot. Results: MGP was expressed rapidly and intensively following exposure to COM and was time-dependent with exposure to oxalate. Conclusion: Our results have given a profile of MGP expression in renal tubular epithelial cells exposed to oxalate and crystals and provided support for an association between MGP and kidney stones. Further investigations on the mechanism of the MGP in the process of crystal-cell interaction may be important in the future. Copyright (C) 2010 S. Karger AG, Basel”
“Purpose: To compare the pattern of protein-expression profiles in blood-plasma after exposure of CBA/CaJ mice to 0 or 3 Gy of Cs-137 gamma rays.

Materials and methods: Two-dimensional electrophoresis gel coupled with mass spectrometry was used to analyze blood-samples collected at days 2 and 7 post-irradiation. At each sacrifice-time,

alterations in expression-level of protein spots between control- and exposed-groups were analyzed statistically by the PDQuest software using Student’s t-test (at the significance level of p<0.05). Mass spectrometry was used to identify the identity of protein-spots with significantly altered expression-level.

Results: At day 2, 18 proteins were significantly up-regulated in exposed-mice. AZD2014 These included: alpha-2-Heremans-Schmid (HS)-glycoprotein, apolipoprotein (Apo)-AII-precursor, Apo-E, beta-2-glycoprotein-I, clusterin, fibrinogen-alpha-chain, fibrinogen-gamma-polypeptide, fetuin-B, haptoglobin, high-molecular-weight (HMW)-kininogen (Kng), low-MW-Kng, Kng1-precursor, selleck chemicals liver-carboxylesterase-I-precursor, major-urinary-protein-6-precursor, mannose-binding-protein-C-precursor,

mannose-binding-lectin-C, and prothrombin-precursor. Gelsolin was detected in control- mice only. At day 7, high expression-levels of 14 proteins were detected in control-mice (i.e., alpha-1-antitrypsin-precursor, carboxylesterase-N, cholesterol-7-alpha-hydroxylase, contraspin, coagulation-factor-II, coagulation-factor-XIII, gelsolin, immunoglobulin-Gheavy-chain, neurexin, prothrombin-precursor, protein-phosphatase, putative-calcium-influx-channel, vitamin-D-binding-protein, and 1110018G07Rik); while 15 proteins were highly expressed in exposed-mice. These included: alpha-1-acid-glycoprotein, alpha-2-HS-glycoprotein, alpha-1-protease-inhibitor-2, ApoA-IV, ApoC-I, ApoH, beta-1-globin, clusterin, complement-component-3, fibrinogen-beta-chain, HMW-Kng, major-histocompatibility-complex-class-Ia-H2-K, serine(cysteine)-proteinase-inhibitor, retinoblastoma-associated-protein-140, and vascular-cell-adhesion-molecule-1.