The placement of a block below the center axis indicates inverted

The placement of a block below the center axis indicates inverted regions. Comparisons between WORiC and WOCauB2 reveal a single block of homologous sequences spanning the structural and packaging regions (figure 3a). There are three separate areas of dissimilarity between WORiC and WOCauB2. These include two transposable elements and an uncharacterized phage protein [WRi_007190]. Notable areas of dissimilarity between WOVitA1 Selleckchem HMPL-504 and WORiC (white areas; figure 3b) include two transposable elements [WRi_006820] interrupting an ankyrin repeat protein gene [WRi_006810, WRi_p06840]. Genome alignments were also used to assign possible functions to

previously annotated hypothetical ORFs. A hypothetical gene, [WRi_p07030], shares 74.7% pairwise identity to the virulence protein gene VrlC.1 of WOVitA1 and is

pseudonized by the transposon insertion [WRi_007040]. The annotated hypothetical protein [WRi _007070] is homologous to tail protein I from WOVitA1 (96%, 3e-143). The major region of dissimilarity between WOVitA1 and WORiC could be a result of horizontal gene transfer into WOVitA1 or gene loss in WORiC. These ORFs in WOVitA1 encode MutL and three transcriptional regulators [ADW80184.1, ADW80182.1 to ADW80179.1]. Although WOVitA1 and WORiC share 36 homologs compared to 33 shared between WORiC and WOCauB2, WORiC is more similar to WOCauB2 (92.4%). The WORiB genome shares only the ORFs found within the packaging region

[WRi_005460 to WRi_005610] with WORiC (figure 3c). However, when the pyocin sequences, containing the viral structural genes, PLX3397 purchase are included in the WOMelB genome and aligned with WORiC, the structural and packaging regions are conserved, but rearranged in WOMelB compared to WORiC (figure 3d). The evolutionary relationships of the tail morphogenesis module and head assembly and DNA packaging module were examined by phylogenetic analysis. Phylogenetic trees based on baseplate assembly protein W and the large terminase subunit showed different evolutionary relationships for related phages, with the exception of the WOMelB, WORiB1 and WORiB2 clade (figure 4). WORiC shows the greatest phylogenetic relatedness Molecular motor to WOCauB2 and WOCauB3 for baseplate assembly protein W (figure 4a), which is reflected by the degree of nucleotide similarity in the alignment (figure 3a). In contrast, the large terminase subunit of WORiC is most closely related to the wMel and wRi B-type phages (figure 4b). Figure 4 Phylogeny of terminase and baseplate assembly protein W amino acid sequences. Maximum-likelihood phylogeny based on translated amino-acid sequences of A) baseplate assembly gene W (tail morphogenesis module) and B) large terminase subunit gene (DNA packaging and head assembly module) of Wolbachia WO phages from published genomes. Bootstrap values for each node are based on 1000 resamplings.

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