Vasopressin administration during cardiopulmonary bypass led to normal capillary density (189.9 +/- 3.9 vs 178.0 +/- 6.3; P=.1) and tissue blood flow (501.7 +/- 39.3 vs 494.7 +/- 44.4 AU; P=.4) compared with baseline. The expression of endothelin-1 (3.2 +/- 0.4 vs 1.8 +/- 0.3 RQ; P=.3) and endothelin subtype A (0.7 +/- 0.2 vs 0.9 +/- 0.2 RQ; P=.5) was not different from the sham group.
Conclusions: Cardiopulmonary Bindarit mouse bypass leads to microvascular impairment of jejunal microcirculation, which is associated with the upregulation of endothelin-1 and endothelin subtype A. The administration of vasopressin minimizes these cardiopulmonary bypass-associated alterations. (J Thorac Cardiovasc
Surg 2013;145:539-47)”
“H/Rouen (displaying a helpless phenotype in the tail suspension test) mice exhibiting features of depressive disorders and NH/Rouen (displaying non-helpless phenotype) mice were previously created through behavioural screening and selective breeding. Learned helplessness (LH), in which footshock stress induces a coping deficit, models some aspects of depression in rodents, but so far, fewer LH studies have been performed in mice than in rats.
To study H/Rouen and NH/Rouen in the LH paradigm.
When CD1 mice were submitted to footshock with various training durations and shock intensities, the
most suitable Idasanutlin parameters to induce a behavioural deficit were 0.3 mA and four training sessions. A significantly longer latency to escape shocks was found in male H/Rouen mice compared to male NH/Rouen mice. On the other hand, once shocked,
NH/Rouen mice showed more severe coping deficits than H/Rouen mice. In addition, a sub-chronic treatment with fluoxetine lacked efficacy in NH/Rouen mice, whereas it improved performances in H/Rouen mice. HAS1 We also found that a shock reminder at day 8, subsequent to inescapable shocks, maintained helplessness for 20 days. Finally, female H/Rouen mice responded to chronic fluoxetine administration after 10 days of treatment, while a 20-day treatment was necessary to improve the behavioural deficit in H/Rouen male mice.
H/Rouen and NH/Rouen lines displayed different despair-related behaviour in the LH paradigm. Fluoxetine had beneficial effects after sub-chronic or chronic but not acute treatment of H/Rouen mice, thus providing a pharmacological validation of the protocols.”
“Objectives: Cardiogenic shock after cardiac surgery is accompanied by a high mortality rate. Early institution of hemodynamic support with a versatile, easy to insert left ventricular assist device might help bridge patients to recovery or to the next therapy, and improve the outcomes.
Methods: Patients developing cardiogenic shock or low cardiac output syndrome after being weaned off cardiopulmonary bypass were enrolled in a prospective single-arm feasibility study (RECOVER I).