Our results indicated that karyoplast-cytoplast interactions and mtDNA haplotype compatibility may affect bovine intersubspecies SCNT efficiency. This study on bovine intersubspecies SCNT is valuable for understanding the mechanisms of mtDNA haplotype compatibility between karyoplast and cytoplast impacting the bovine SCNT efficiency, and provides an alternative and economic resource for HID cloning.”
“Copper-encapsulated polystyrene nanocomposite

particles were prepared through ex situ dispersion of Cu nanoparticles into monomer droplets and subsequent polymerization using water in supercritical carbon dioxide (water-in-sc-CO2) at 70 degrees C. First, colloidal click here dispersion of copper nanoparticles was synthesized by chemical reduction of copper chloride (CuCl2) using sodium borohydrate (NaBH4) as reducing agent. Colloidal dispersion of copper nanoparticles was added slowly during the polymerization of styrene using water-in-sc-CO2 medium at 70 degrees C and 20.68 MPa. Cu nanoparticle encapsulated polymer particles were characterized

by UV, X-ray diffraction, thermograyimetric analysis, SEM, and TEM. Cu nanopartides were uniformly distributed inside the polymer matrix during the polymerization process. This work represents a simple way to prepare a variety of metal nanoparticles encapsulated polymer particles using waterin-sc-CO2 medium. The Cu/polystyrene nanocomposite particles exhibit antimicrobial activity against a number of bacteria. The current work represents a simple, cheap and universal way to selleck prepare a variety of metal polymer nanocomposite materials. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 120: 1027-1033, 2011″
“The aim of this study is to optimize the different stages for the obtaining of chondroitin sulphate from skate cartilage by means of experimental designs and kinetic approaches. The results of the

hydrolysis cartilage using papain and an Apoptosis inhibitor enzymatic preparation from ray pancreas are compared. Moreover, the basic differences among enzymatic and chemical hydrolysis are identified. In order to reduce the aggressive treatments with strong alkalis a combined step of hydrolysis-precipitation in hydroalcoholic solution is also proposed. Thus, the combination of enzymatic and chemical hydrolysis, selective precipitation together with membranes technology lead to the formulation of a quick and highly efficient process (15%, w/w) with low consumption of reagents and high purity for the chondroitin sulphate (I=99%) obtained. (C) 2009 Elsevier B.V. All rights reserved.”
“Lesion mimic is necrotic lesions on plant leaf or stem in the absence of pathogenic infection, and its exact biological mechanism is varied.