“We report super-resolution imaging in a metamaterial syst


“We report super-resolution imaging in a metamaterial system comprising spherical silver nanoparticle chain arrays, where each chain consists of nanoparticles with a smaller particle added to the end. Our simulations reveal that silver nanoparticale chains

have subwavelength resolution capability at visible wavelengths and that the field intensity in the imaging selleck inhibitor plane varies with the number of layers of nanoparticles, their polarization, and their coupling. By adding a smaller nanoparticle at the end of each chain, the resolution capability is significantly enhanced, and high-quality super-resolution imaging can be realized for incident waves polarized along the chain direction. VC 2011 American Institute of Physics. [doi:10.1063/1.3553875]“
“Previous studies of highly branched mutants in pea (rms1-rms5), Arabidopsis thaliana (max1-max4), petunia (dad1-dad3), and rice (d3, d10, htd1/d17, d14,

d27) identified strigolactones or their derivates (SLs), as shoot branching inhibitors. This recent discovery offers the possibility of using SLs to regulate branching commercially, for example, in chrysanthemum, an important cut flower crop. To investigate this option, SL physiology and molecular biology were studied in chrysanthemum (Dendranthema buy AS1842856 grandiflorum), focusing on the CCD8/MAX4/DAD1/RMS1/D10 gene. Our results suggest that, as has been proposed for Arabidopsis, the ability of SLs to inhibit bud activity depends

on the presence of a competing auxin source. The chrysanthemum SL biosynthesis gene, CCD8 was cloned, and found to be regulated in a similar, but not identical way to known CCD8s. Expression analyses revealed that DgCCD8 is predominantly expressed in roots and stems, and is up-regulated by exogenous auxin. Exogenous SL can down-regulate DgCCD8 expression, but this effect can be overridden by apical auxin application. This study provides evidence see more that SLs are promising candidates to alter the shoot branching habit of chrysanthemum.”
“PRINCIPLES: Reimbursement for inpatient treatment in Switzerland is in transition. While hospitals in some cantons already use Diagnosis Related Groups (DRG) based systems for hospital financing, others use fee-for-service (FFS) based systems, a situation that provides the opportunity to perform a head-to-head comparison between the two reimbursement systems. The aim of this analysis was to compare reimbursement systems with regard to length of hospital stay (LOS) and patient outcomes in a cohort of community-acquired pneumonia patients from a previous prospective multicentre study in Switzerland.

METHODS: This is a post-hoc analysis of 925 patients with community-acquired pneumonia from a previous randomised-controlled trial.

To monitor global gene expression, the transcriptomes of bone mar

To monitor global gene expression, the transcriptomes of bone marrow-derived MSCs expanded under serum-free and serum-containing conditions were find protocol compared, revealing similar expression profiles. In addition, the described serum-free culture medium supported the isolation of human MSCs from primary human marrow aspirate with continual propagation.

Conclusions: Although the described serum-free MSC culture medium is not free of xenogeneic components, this medium provides a substitute for serum-containing medium for research applications, setting the stage for future clinical applications.”
“The inflammatory

and immune responses evoked in sepsis may create not only an acute brain dysfunction, which occurs in the majority of septic patients, but also long-term deficits such as memory impairment. In this context, we evaluated depressive-like parameters in sepsis survivor rats. For this purpose, male Wistar rats, NU7441 mw weighing 300-350 g, underwent cecal ligation and perforation (CLP) (sepsis group) followed by “”basic support”", or were sham-operated (control group). After 3 days of the sepsis procedure, the animals were treated with imipramine at 10 mg/kg or saline during 14 days (days 3-17). The consumption of sweet food was measured for 7 days (days 10-17) and the body weight was measured

before CLP, 10, and 17 days after CLP. Seventeen days after sepsis (immediately after sweet food consumption measurement), the animals were anesthetized and blood was withdrawn for the analyses of corticosterone and adrenocorticotropic hormone (ACTH) levels, and immediately killed by decapitation. The adrenal gland and hippocampus were immediately isolated and weighed, and the hippocampus was utilized for determining brain-derived neurotrophic factor (BDNF) levels. It was observed that animals subjected to CLP presented decreased sucrose intake. Epigenetics inhibitor Septic rats did not increase body weight and presented an increase in the weight of adrenal gland. Both corticosterone and ACTH levels were increased, while hippocampus

weight and BDNF levels in the hippocampus decreased. The treatment with imipramine reversed all the parameters described above. Our results supported the hypothesis that rats that survive sepsis show depressive-like behavior, alterations in the hypothalamus-pituitary-adrenal axis, and decreased BDNF levels in the hippocampus.”
“PURPOSE: To compare postoperative inflammation in patients receiving 1 of 3 AcrySof intraocular lenses (IOLs): MA60AC (Group 1), SA60AT (Group 2), or SN60AT (Group 3).

SETTING: Service d’Ophtalmologie, Universite Paris Descartes Hopital Cochin, Paris, France.

METHODS: This prospective randomized 3-month study included eyes that received 1 of the 3 IOL models with standard surgery and postoperative care. Anterior chamber cells were assessed at the slitlamp and anterior chamber flare values, with a Kowa 500 flare meter.

RESULTS: Fifty-nine patients (59 eyes) with a mean age of 72.7 years were evaluated.

The Mann-Whitney U-test and Friedman’s anova were used for the st

The Mann-Whitney U-test and Friedman’s anova were used for the statistical treatment of data.

ResultsVAS, sedation scores and nausea/vomiting scores were

similar in both groups (P>0.05). The 24-h tramadol consumption was significantly lower in group I (420.1566.58mg) than in group II (494.00 +/- 29.45mg), while patient satisfaction was significantly higher in group I (P<0.05).

ConclusionWhile tramadol administration by either of the methods used may ensure efficient early postoperative anesthesia in cesarean section patients, i.v. PCA may be preferred because of the lower drug consumption and higher patient satisfaction associated with it.”
“Transurethral resection (TUR) syndrome, resulting from dilutional hyponatraemia for excessive absorption of irrigating fluid, represents the most relevant complication of transurethral resection of prostate (TURP). Ethanol is used as a tracer in the irrigant solution check details to monitor fluid absorption with a breathalyser. An unusual case of transient acute liver failure complicating TUR syndrome is reported. A 54-year-old male patient, without risk factors for the development of selleck kinase inhibitor toxic hepatitis, was subjected to TURP for treatment of benign prostatic hyperplasia. Fluid absorption (2275 ml), estimated by breathalyser, exceeded maximum allowed absorption (2000 ml) only at the end of the surgical intervention. No signs of possible toxicity were evident

in the few hours following the intervention. About 10 h after the end of TURP, the patient developed

sweating, vomiting and diarrhoea. Laboratory analysis revealed severe hyponatraemia (116 meq/l) with signs of severe liver impairment (total bilirubin 5.8 mg/dl, alanine aminotransferase 56 500 U/l, aspartate aminotransferase 32 700 U/l), kidney failure (serum creatinine 1 93 mg/dl) and serum ethanol levels of 219 mg/dl (0.2%). The patient was treated with acetylcysteine 150 mg/kg i.v. and furosemide 50 mg i.v. Liver and renal functions improved in few days and recovered completely within 30 days. The TUR syndrome observed in this case was probably extravascular in nature, and could have been identified and prevented by measuring ethanol levels PKC412 solubility dmso 10 min after ending the surgical procedure. The performance of such a test should be strongly recommended to all surgeons. The clinicians attributed the development of liver impairment in this case to ethanol toxicity. However, further studies are warranted to confirm whether hepatic injury can represent a possible complication of TUR syndrome when ethanol solution is used as irrigant fluid.”
“Purpose of review

Previous studies on rehabilitation following total knee arthroplasty (TKA) demonstrated limited efficacy in increasing long-term outcomes. More recently, several rehabilitation approaches have demonstrated greater efficacy for increasing long-term strength and functional performance outcomes following TKA.


“Purpose of review

The current review addresses


“Purpose of review

The current review addresses a critical need in clinical islet transplantation, namely the routine transition from the requirement of two to four donors down to one donor per recipient. The ability to achieve single-donor islet transplantation will provide many more islet grafts for treatment of an ever-expanding patient base with type 1 diabetes (T1DM) with poor glycemic control. Avoiding exposure of recipients to multiple

different donor human leukocyte associated (HLA) antigens is critical if risk of donor sensitization is to be avoided. This point is important as further islet or pancreas transplants HDAC inhibitor in the remote future or the potential future need for a solid organ kidney transplant may become prohibitive if the recipient is sensitized.

Recent findings

This review addresses systematically all areas that contribute to the success or failure of single-donor islet engraftment, beginning with donor-related factors, optimizing islet

isolation and culture conditions, and describes a series of strategies in the treatment of the recipient to prevent inflammation, apoptosis, islet thrombosis, and improve metabolic functional outcome, all of which will lead to improved single-donor engraftment success.

Summary

If single-donor islet transplantation can be achieved routinely, therapy will become more widely available, more accepted by the transplant community (currently pancreas transplantation requires only a single donor), and this situation will have a major impact overall as an effective treatment option in T1DM.”
“OBJECTIVE: To assess the cost-effectiveness

EGFR inhibitors cancer of diagnostic laparoscopy, selleck screening library computed tomography (CT), and magnetic resonance imaging (MRI) after indeterminate ultrasonography in pregnant women with suspected appendicitis.

METHODS: A decision-analytic model was developed to simulate appendicitis during pregnancy taking into consideration the health outcomes for both the pregnant women and developing fetuses. Strategies included diagnostic laparoscopy, CT, and MRI. Outcomes included positive appendectomy, negative appendectomy, maternal perioperative complications, preterm delivery, fetal loss, childhood cancer, lifetime costs, discounted life expectancy, and incremental cost-effectiveness ratios.

RESULTS: Magnetic resonance imaging is the most cost-effective strategy, costing $6,767 per quality-adjusted life-year gained relative to CT, well below the generally accepted $50,000 per quality-adjusted life-year threshold. In a setting where MRI is unavailable, CT is cost-effective even when considering the increased risk of radiation-associated childhood cancer ($560 per quality-adjusted life-year gained relative to diagnostic laparoscopy). Unless the negative appendectomy rate is less than 1%, imaging of any type is more cost-effective than proceeding directly to diagnostic laparoscopy.

CONCLUSIONS: Depending on imaging costs and resource availability, both CT and MRI are potentially cost-effective.

The search terms were benign estimation of alpha one blockers, de

The search terms were benign estimation of alpha one blockers, determination of prazosin, terazosin, tamsulosin, doxazosin, alfuzosin. Similarly HPLC and Spectroscopy methods for estimation of prazosin, terazosin, tamsulosin, doxazosin, alfuzosin.

Results: Total 57 analytical methods were found. 11 spectrophotometry, 39 chromatographic methods including 2 ESI MS/MS, 1 ESI MS, 2 HPTLC, 3 TLC, 17 HPLC with UV, 9 with fluorescence, 1 with PF-03084014 purchase electrochemical detection, 4 with MS detector. Other than this 3 voltametry, 1 method each for radioreceptor assay, polarography, capillary electrophoresis and potentiometry titrations were found.

Conclusion: Radioreceptor assay, LC-MS, UPLC-MS

methods were found to be the most sensitive GSK1120212 concentration for prazosin, terazosin and doxazosin respectively. Likewise, HPLC-MS-MS methods was found as most sensitive method for estimation of tamsulosin and alfuzosin.”
“Study Design. Spine proprioception and postural control in unstable sitting were compared in 18 chronic low back pain patients using a repeated measures design.

Objective. The study objective was to determine if stochastic resonance (SR) stimulation of the paraspinal muscles improves spine proprioception

and trunk postural control.

Summary of Background Data. Decreased spine proprioception and larger postural sway have been found in low back pain patients, although several studies have also shown no differences in spine proprioception.

Methods. Spine proprioception, measuring subjects’ sensitivity to change in position, was assessed in 3 orthopaedic planes. Postural control was assessed using an unstable seat with a hemisphere attached to the bottom. Subjects balanced with eyes closed on the most challenging size hemisphere they could manage while center-of-pressure

was recorded with a force plate beneath the seat. Both tasks were performed with SR stimulation randomized at 0%, 25%, 50%, and 90% intensity levels.

Results. No significant differences in spine proprioception were observed between SR stimulation levels for any of the 3 orthopaedic planes. SR stimulation significantly improved postural control, but only in the lateral plane. No differences QNZ molecular weight in postural control were observed between stimulation levels 25%, 50%, and 90% in the lateral plane. There was no correlation between spine proprioception and postural control.

Conclusion. Results suggest that SR stimulation to the paraspinal muscles can improve postural control; however, this improvement cannot be attributed to improved spine proprioception based on the current study. People with compromised neuromuscular control or those exposed to unstable environments may benefit from SR stimulation.”
“Purpose of review

The kidney has an elaborate and complicated structure comprising several cell types.

These data provide general benchmark rates for NND with spine sur

These data provide general benchmark rates for NND with spine surgery as a basis for patient counseling and for ongoing efforts to improve safety of care.”
“Eucalyptus is a genus widely cultivated in many tropical and subtropical regions of the world as one of the main sources of raw materials for the pulp and paper industry. Identification of clones and selection of genotypes with desirable agronomic characteristics would be useful. We assessed eucalyptus full-sibs that varied in wood quality, using a combination of two-dimensional gel electrophoresis and mass spectrometry to identify differentially expressed proteins as candidates for quality markers. Thirty-one differently expressed proteins were identified, including three

proteins of clone X1, four of clone X2, and 12 each of clones X3 and X4. These proteins are involved in various biological processes, including polyphosphate biosynthesis, catalytic activity, nucleotide excision repair, cellular Selleckchem Pinometostat metabolic processes, cell redox homeostasis, response to salt stress, response to temperature, oxidation and reduction processes, cellular water homeostasis, and protein phosphorylation. In the cambial region of each clone, the proteins ketol-acid reductoisomerase, uncharacterized protein MG428, receptor-like serine/threonine-protein kinase and a heat

shock protein were found in larger quantities in clone X4 than in clone X1. These proteins are known to be related to protection against oxidative stress and biosynthesis selleck products of lignin. A high buildup of proteins involved in response to stress in the cambial region of eucalyptus would indicate Duvelisib manufacturer clones with undesirable characteristics for use in the pulp and paper industry.”
“Purpose: The aim of this study was to examine the effect of extremely low-frequency magnetic fields (MF) and the endocrine-disrupting compound atrazine, each separately, on the thyroid gland of juvenile-peripubertal rats, and to investigate the possible synergistic effect of these two factors combined.

Materials and methods: The study was performed on male Wistar rats from postnatal day 23-53. Animals were divided into six groups: (1) 4 h/day exposure to

MF (50 Hz, 100-300 mu T, 54-160 V/m), (2) 20 mg/kg of body weight (bw) of atrazine, (3) 200 mg/kg bw of atrazine, (4) MF with 20 mg/kg bw of atrazine (5) MF with 200 mg/kg bw of atrazine, and (6) control.

Results: Light and electron microscopic studies demonstrated no significant alterations in the thyroid structure between the treated groups and the control. Significant outcomes were found regarding the volume density of thyroid follicles and the connective tissue between the MF-exposed group when compared to both atrazine treatments and the combined treatments. The high dose of atrazine significantly affected the number of mast cells compared to the control.

Conclusions: No synergistic effect of the MF and the endocrine-disrupting compound atrazine on the thyroid gland has been found.

Furthermore, the tunneling

of spin-polarized charge carri

Furthermore, the tunneling

of spin-polarized charge carriers between SB273005 clinical trial the YBCO layers through LSMO showed sharp switching between the high and low resistance states, which we assume corresponds to antiparallel and parallel alignments of the magnetic (spin) moments of these hybrid structures. The investigation of these promising hybrid structures is aimed at understanding the science of the new spin-based devices, laying the foundation for a new generation of ultrafast, nonvolatile electronics. (C) 2009 American Institute of Physics. [DOI: 10.1063/1.3076544]“
“Objectives: The knee can be injected at different anatomic sites with or without image-guidance. We undertook a systematic review to determine the accuracy of intra-articular knee injection (IAKI) and whether this varied by site, use of image-guidance, and experience of injectors, and whether accuracy of injection, site, or use of image-guidance influenced outcomes following IAKIs.

Methods: Medline, Embase, AMED, CINAHL, Web of Knowledge, Cochrane Central Registers for Controlled Trials up to Dec 2012 were searched

for studies that evaluated either accuracy of IAKIs or outcomes related to accuracy, selleck products knee injection sites, or use of image-guidance. Within-study and between-study analyses were performed.

Results: Data from 23 publications were included. Within-study analyses suggested IAKIs at the superomedial patellar, medial midpatellar (MMP), superolateral patellar (SLP) and lateral suprapatellar bursae sites were more accurate when using image-guidance than when blinded (ranges of pooled risk difference 0.09-0.19). Pooling data across check details studies suggested blinded IAKIs at the SLP site were most accurate (87%) while MMP (64%) and anterolateral joint line (ALJL) sites were (70%) least accurate. Overall about one in five blinded IAKIs were

inaccurate. There was some evidence that experience of the injector was linked with improved accuracy for blinded though not image-guided injections. Based on a small number of studies, short but not longer-term outcomes for ultrasound-guided were found to be superior to blinded IAKIs.

Conclusions: Image-guided IAKIs are modestly more accurate than blinded IAKIs especially at the MMP and ALJL sites. Blinded injections at SLP site had good accuracy especially if performed by experienced injectors. Further studies are required to address the question whether accurate localization is linked with an improved response. (C) 2013 The Authors. Published by Elsevier HS Journals, Inc. All rights reserved.”
“A cylindrical probe with almost perfectly flat plateaulike surface was focused ion beam (FIB) milled from an atomic force microscopy probe in order to create the required surface conditions for thin film deposition with finely controlled deposition/growth parameters.

Among DNA samples from 135 Brazilian Zebu breed cattle, we identi

Among DNA samples from 135 Brazilian Zebu breed cattle, we identified an RFLP/HpaII polymorphism (T/C), which, based on a restriction methylation-sensitive assay employing HpaII

and isoschizomer MspI enzymes (methylation-sensitive and -non-sensitive enzymes, respectively), appears to be a DNA methylation point. This is the first report of this polymorphism and on DNA methylation in the bovine CYP21 PI3K inhibitor promoter region in Brazilian Zebu cattle.”
“Capacitively coupled plasmas (CCPs) generated using high frequency (3-30 MHz) and very high frequency (30-300 MHz) radio-frequency (rf) sources are used for many plasma processing applications including thin film etching and deposition. When chamber dimensions become commensurate with the effective rf wavelength in the plasma, electromagnetic wave effects impose a significant influence on plasma behavior. Because the effective rf wavelength in plasma depends upon both rf and plasma process conditions (e.g., rf power and gas pressure), a self-consistent model including both the rf power delivery system and the plasma discharge is highly desirable to capture a more complete physical picture of 4-Hydroxytamoxifen the plasma behavior. A three-dimensional model for self-consistently studying both electrodynamic and plasma dynamic behavior of large-area (Gen 10, >8 m(2)) CCP is described in this

paper. This model includes Maxwell’s equations and transport equations for charged and neutral species, which are coupled and solved in the time domain. The complete rf plasma discharge chamber including the rf power delivery subsystem, rf feed, electrodes, and the plasma domain is modeled as an integrated system. Based on this full-wave solution model, important limitations for processing uniformity imposed by electromagnetic wave propagation effects in a large-area CCP (3.05 x 2.85 m(2) electrode size) are studied. The behavior of H-2 plasmas in such a reactor is examined from 13.56 to 200 MHz. It is shown that various rectangular harmonics of electromagnetic

Nutlin-3 clinical trial fields can be excited in a large-area rectangular reactor as the rf or power is increased. The rectangular harmonics can create not only center-high plasma distribution but also high plasma density at the corners and along the edges of the reactor. (C) 2010 American Institute of Physics. [doi:10.1063/1.3489950]“
“The efficacy of random primer-pair arrays compared to conventional RAPD method with a single decamer primer was evaluated using DNA from two species of Cucumis. The banding patterns of amplicons revealed enhanced utility of primer-pair arrays over conventional RAPDs, producing more bands and a higher degree of polymorphism, both at intra- and inter-specific levels. Amplification produced by both methods clearly distinguished a wild from a cultivated species of the genus Cucumis.

5% of the urologists and biomarkers by 16 3% Preoperative models

5% of the urologists and biomarkers by 16.3%. Preoperative models were used by 20.9%, postoperative models by 38.4%,

and metastatic models by 38.4%. The Raj and Motzer models were the most used in preoperative and metastatic settings, while no predominance among the different postoperative models was seen. The most important reasons to skip the use of models were “”lack of additional value” and “”lack of familiarity” reported by 30.2% and 27.9% of the responders, respectively.

Conclusions: The TNM is the mainstay for assessing prognosis in RCC. Our data indicate that penetration of prognostic systems is, at most, moderate, suggesting limited use outside original developmental settings. On the contrary, clinical, laboratory, and pathologic factors are used by almost all urologists click here for prognosis estimations. The most important reason not to use models is the lack of additional value.”
“Methylphenidate (MPH) is a commonly abused psychostimulant prescribed for the treatment of attention deficit hyperactivity disorder. MPH has a mechanism of action similar to cocaine (COC) and is commonly SRT1720 solubility dmso characterized as a dopamine transporter (DAT) blocker. While there has been extensive work aimed at understanding dopamine (DA) nerve terminal changes following COC

self-administration, very little is known about the effects of MPH self-administration on the DA system. We used fast scan cyclic voltammetry LCL161 in nucleus accumbens core slices from animals with a 5-day self-administration history of 40 injections/day of either MPH (0.56mg/kg) or COC (1.5mg/kg) to explore alterations in baseline DA release and uptake kinetics as well as alterations in the interaction of each

compound with the DAT. Although MPH and COC have similar behavioral effects, the consequences of self-administration on DA system parameters were found to be divergent. We show that COC self-administration reduced DAT levels and maximal rates of DA uptake, as well as reducing electrically stimulated release, suggesting decreased DA terminal function. In contrast, MPH self-administration increased DAT levels, DA uptake rates and DA release, suggesting enhanced terminal function, which was supported by findings of increased metabolite/DA tissue content ratios. Tyrosine hydroxylase messenger RNA, protein and phosphorylation levels were also assessed in both groups. Additionally, COC self-administration reduced COC-induced DAT inhibition, while MPH self-administration increased MPH-induced DAT inhibition, suggesting opposite pharmacodynamic effects of these two drugs. These findings suggest that the factors governing DA system adaptations are more complicated than simple DA uptake blockade.”
“Aim: Premenstrual syndrome (PMS) is a commonly encountered complaint among women. It may affect women’s quality of life and reduce their occupational productivity.

A total of 522 male and 561 female adolescents were enrolled into

A total of 522 male and 561 female adolescents were enrolled into the final analyses. Anthropometric parameters, blood pressure, blood biochemistry, fasting insulin, and plasma sRAGE levels were measured.

In males, sRAGE was significantly and inversely correlated with waist circumference (WC), body mass index (BMI), systolic blood pressure, triglyceride (TG), low density lipoprotein cholesterol (LDL-C), and homeostasis model assessment-insulin resistance (HOMA-IR). Only WC and BMI were significantly and inversely HSP990 correlated with sRAGE in females. Using linear regression analysis adjusting for age and gender, significant association was found between sRAGE and WC, BMI, TG, LDL-C, and HOMA-IR in adolescents of either gender (P < 0.05). This association was abolished when further adjusting BMI. In addition, sRAGE was significantly and inversely correlated with the increasing number of components of MetS in males 3-MA (P for trend = 0.006) but not in females (P for trend = 0.422). In conclusion, plasma sRAGE is associated with

obesity and MetS among adolescents. BMI may be the most important determinant of sRAGE levels in adolescents.”
“We examined the antibacterial activities of several types of propolis, including Africanized honey bee green propolis and propolis produced by meliponini bees. The antibacterial activity of green propolis against Micrococcus luteus and Staphylococcus aureus was superior to that of Melipona

quadrifasciata and Scaptotrigona sp propolis. Only two samples of propolis (green propolis and Scaptotrigona sp PF-00299804 solubility dmso propolis) were efficient against Escherichia coli. Melipona quadrifasciata propolis was better than green propolis and Scaptotrigona sp propolis against Pseudomonas aeruginosa. We concluded that these resins have potential for human and veterinary medicine.”
“Hydrogen ions were implanted into 500-nm-thick Pb(Zr, Ti)O-3 ferroelectric thin films with different doses and energies. An antiferroelectric behavior was confirmed in the implanted thin films with proper H+ injection energies from independent measurements of polarization-electric hysteresis loops and capacitance-voltage curves. With the increase in the H+ doping concentration and implanting energy up to 25 keV, the characteristic pseudoantiferroelectric behavior becomes more evident in the films along with the concomitant reduction in the remnant polarization. However, the above antiferroelectricity is weakened for the restoration of a preferred ferroelectric state, once the implanting energy is higher than 35 eV.