The expression patterns of Pax6, Wnt8, and HoxA2 genes in early e

The expression patterns of Pax6, Wnt8, and HoxA2 genes in early embryos of S. canicula showed close correspondence to what has been described in other vertebrates and helped to identify the anterior rhombomeres. Also in these early embryos, the combination of Pax6 with protein markers of migrating neuroblasts (DCX) and early differentiating neurons

(general: HuC/D; neuron type specific: GAD, the GABA synthesizing enzyme) revealed the organization of S. canicula hindbrain in both transverse segmental units corresponding to visible rhombomeres and longitudinal columns. Later in development, when the interrhombomeric Metabolism inhibitor boundaries fade away, accurate information about S. canicula hindbrain subdivisions was achieved by comparing the expression patterns of Pax6 and GAD, serotonin (serotoninergic neurons), tyrosine hydroxylase (catecholaminergic neurons), choline acetyltransferase (cholinergic neurons), and calretinin (a calcium-binding protein). The patterns observed revealed many topological correspondences with other vertebrates and led to reconsideration of the current view of the elasmobranch hindbrain segmentation

as peculiar among vertebrates.”
“The quantitative analysis of thin layers using Heavy Ion-Elastic Recoil Detection (HI-ERD) can be reliably performed if the stopping powers of the probing ions and recoils in a given target matrix are known accurately. Unfortunately for many projectile/target combinations experimental data is limited and Rigosertib cell line where available, deviations of up to 50% between experiment and theory have been reported. This presentation describes JQ1 in vivo the assembly of a Time of Flight-Energy (ToF-E) detector system developed for HI-ERD analysis and adapted for stopping power measurements at iThemba LABS.

First results from energy loss measurements of 0.1-0.5 MeV/nucleon (28)Si and (84)Kr ions in ZrO(2) are presented and compared with predictions of the widely used SRIM2003 (Stopping Range of Ions in Matter). (C) 2009 Elsevier B. V. All rights reserved.”
“The molecular and biochemical bases of fertility restoration were explored using cytoplasmic male sterile (CMS) Ji A, maintainer Ji B, and two cotton hybrids RF1 and QF(1), developed by crossing CMS with DES-HAF277 (normal restorer) and Zheda strong restorer (transgenic restorer with GST gene), respectively. Transcript levels of both exogenous and endogenous GST genes were high in anther as compared to other plant tissues of the QF(1) hybrid. Moreover, the expression of the GST gene during meiosis (stage 2) and microspore development (stage 3) was highest in the QF(1) hybrid. The ratio of cyanide-resistant respiration to total respiration was also high in the QF(1) hybrid during stage 2 and stage 3 as compared to the RF1 hybrid. O-2(-) and H2O2 contents increased more during stage 2 in the CMS line and stage 3 in the RF1 hybrid compared to the maintainer and QF1 hybrid.

Mean vertical velocities calculated from high-resolution dive dat

Mean vertical velocities calculated from high-resolution dive data (62 dives > 150 m) from three individuals suggested that mantas may use gliding behavior during travel and that this behavior may prove more efficient than continuous horizontal swimming. The behaviors in this study indicate manta rays provide a previously unknown link between the epi- and mesopelagic layers of an extremely oligotrophic marine environment and

Galardin inhibitor provide evidence of a third marine species that utilizes gliding to maximize movement efficiency.”
“Background and objective. – Only a few studies have examined the putative association between substance use and bullying others, and to our knowledge none of the previous studies have investigated substance abuse among victims or those who are both bullies and victims. The aim of our study was to Copanlisib solubility dmso investigate substance use among all of these three bullying subgroups and to examine the putative association between substance use and bullying behaviour.\n\nMethods. – The study sample consisted of 508 Finnish adolescents (age 12-17) admitted to psychiatric inpatient care between April 2001 and March 2006. We used the Schedule for Affective Disorder and Schizophrenia for School-Age Children Present

and Lifetime (K-SADS-PL) to obtain DSM-IV psychiatric diagnoses, information about bullying behaviour and substance abuse of the adolescents. JNK-IN-8 chemical structure The level of nicotine dependence (ND) was assessed by using the modified Fagerstrom Tolerance Questionnaire (mFTQ).\n\nResults. – Our study showed

that both among boys and girls, regular daily smoking and alcohol use among boys were statistically significantly associated with bullying behaviour. Further, among girls, but not among boys, there was also an association between bullying behaviour and more severe substance use, such as ND, use of cannabis and hard drugs.\n\nConclusion. – Since an association between bullying and severe substance use was found in this study, our novel finding needs replication in adolescent general population samples. (C) 2010 Elsevier Masson SAS. All rights reserved.”
“According to the concept of pollination syndromes, floral traits reflect specialisation to a particular pollinator or set of pollinators. However, the reproductive biology of endemic, and often specialised, plants may require increased attention as climate change accelerates worldwide. Species of Roscoea endemic to the Himalayan region have striking orchid-like flowers with long corolla tubes, suggesting pollination by long-tongued insects. Until now, the reproductive biology of species of Roscoea has been poorly documented. We investigated the floral biology, breeding system and pollination ecology of R. cautleoides and R.

Three individuals had nephrolithiasis, and three had a history of

Three individuals had nephrolithiasis, and three had a history of exercise-induced acute renal failure. In conclusion, homozygous loss-of-function

mutations of GLUT9 cause a total defect of uric acid absorption, leading to severe renal hypouricemia complicated by nephrolithiasis and exercise-induced acute renal failure. In addition to clarifying renal handling of uric acid, our findings may provide a better understanding of the pathophysiology CUDC-907 mouse of acute renal failure, nephrolithiasis, hyperuricemia, and gout.”
“Efficient Cu-doped InP quantum dots (Cu:InP d-dots) emitters were successfully synthesized by epitaxial growth of a ZnSe diffusion barrier for the dopants. The Cu dopant emission of the Cu:InP/ZnSe core/shell d-dots covered the important red and near-infrared (NIR) window for biomedical applicaitons, from 630 to 1100 nm, by varying the size of the InP host nanocrystals. These new d-dots emitters not only compensate for the emission wavelength of the existing noncadmium d-dots emitters, Cu- and Mn-doped ZnSe d-dots

(450-610 nm), but also offer a complete series of efficient nanocrystal emitters based on InP nanocrystals. The one-pot synthetic scheme for the formation of Cu:InP/ZnSe core/shell cl-clots was successfully established by systematically studying the doping process, the dopant concentration-dependent photophysical properties, and the dopant diffusion during shell epitaxy, etc. Complete elimination of InP bandgap emission BKM120 in vitro and efficient pure dopant emission (with photoluminescence quantum yield as high as between 35-40%) of the core/shell d-dots were achieved

by optimizing the final doping level and the diffusion barrier thickness.”
“We have previously found increased expression of thromboxane synthase (TXAS) and thromboxane receptor (TP) beta isoform in the tissues of patients with bladder cancer. Studies in cell lines and mice have indicated a potential significant role of the thromboxane signaling pathway in the pathogenesis of human bladder cancer. This study was designed to determine if the changes observed in the tissues of patients with bladder cancer were mirrored by changes Rapamycin in the urine of these patients. We found increased levels of thromboxane B-2 (TXB2) the major metabolite of TXAS and increased levels of the TP beta receptor. These results raised the possibility that patients with bladder cancer may be followed for progression or remission of their disease by quantitation of these substances in their urine. (C) 2011 Elsevier Inc. All rights reserved.”
“Neuroendoscopy is a promising therapeutic option for spontaneous intracerebral hemorrhage (ICH). We sought to compare the clinical outcomes between neuroendoscopic surgery and craniotomy for spontaneous ICH. We retrospectively analyzed the clinical and radiographic data of 43 patients treated with 23 neuroendoscopic procedures (endoscopy group) and 20 microsurgical procedures (craniotomy group).

The displacements and stiffness of the three regions were extract

The displacements and stiffness of the three regions were extracted separately and compared between groups to further develop the diagnostic criteria.\n\nThe results indicated that the laxity GNS-1480 behavior was mostly affected in Region 2 and Region 3 after ACL tear. Two good indicators for ACL tear were found in the receiver operating characteristic (ROC) curve analysis: Region 2 with the displacement larger than 3.7 mm and Region 3 with the stiffness smaller than 22 N/mm. These two criteria provided a better diagnostic accuracy with increased sensitivity.\n\nThe regional analysis method developed in this study could provide more information for understanding the characteristics of the anterior knee

laxity and help increase the diagnostic accuracy for ACL rupture.\n\nII.”
“By using a high-speed camera, this paper presents characteristics of plasma-generated KU-57788 research buy bubble oscillation. Multibubbles can significantly affect each other to their oscillation period and acoustic outputs.”
“I isolated and characterized 8 polymorphic anonymous nuclear loci from the federally endangered Mitchell’s satyr butterfly, Neonympha mitchellii mitchellii (Lepidoptera: Nymphalidae). All loci reported here are polymorphic and will serve as important tools in the conservation of this rare butterfly.”
“Homeobox (HOX) family genes, major transcription

factors for embryonic development, have been also implicated in vascular development and angiogenesis, particularly with regulation of genes involved in cell-cell or cell-extracellular matrix (ECM) interactions. However, the cellular and molecular functions of HOXD1 in endothelial cells (ECs) are yet to be explored. We here report that HOXD1 is prominently expressed in human ECs and regulates angiogenic activities. Knockdown of HOXD1 in ECs resulted in significant

inhibition of migration and adhesion as well as tube like structure formation. These effects were correlated with the reduced expression of integrin beta 1 (ITGB1), an important Barasertib nmr signaling component of angiogenesis. Consistently, ITGB1 promoter activity was decreased by HOXD1 knockdown in ECs. Furthermore, we identified the putative HOXD1-binding sites in the promoter region of ITGB1. Together, these findings suggest that HOXD1 plays a significant role in EC functions by regulating the expression of ITGB1. (C) 2011 Elsevier Inc. All rights reserved.”
“Bacteroidetes are commonly assumed to be specialized in degrading high molecular weight (HMW) compounds and to have a preference for growth attached to particles, surfaces or algal cells. The first sequenced genomes of marine Bacteroidetes seemed to confirm this assumption. Many more genomes have been sequenced recently. Here, a comparative analysis of marine Bacteroidetes genomes revealed a life strategy different from those of other important phyla of marine bacterioplankton such as Cyanobacteria and Proteobacteria.

14 U/mg, respectively Noticeably, the protein displayed by pELMO

14 U/mg, respectively. Noticeably, the protein displayed by pELMO was lower than

the protein displayed by pENVO. It can be concluded that Lpp-OmpA can display less protein, but more functional OPH protein. These results highlight the high potential, of both engineered bacteria, for use in the bioremediation of pesticide-contaminated sources in the environment.”
“Benthic suspension feeders are abundant in Mediterranean coastal environments, though most of them are threatened due to overexploitation, climate change impacts, and unregulated diving or fishing practices. Little is known about most of the coastal communities in terms of large-scale distribution and realistic MEK inhibitor benthic-pelagic coupling implications, which are keys to understand and manage those threatened ecosystems. The active suspension feeder Halocynthia papillosa (one of the most common ascidian species of the Mediterranean Sea) was selected as a model organism PD173074 datasheet to help to understand the ecological role in benthic-pelagic coupling processes and its importance as a carbon sink (an essential ecosystem service). The spatial and bathymetrical distribution of this organism has been studied using remotely operated vehicle video transects. The species was distributed throughout the study area, with a maximum density of 4 specimens m(-2). The highest

abundances and the biggest sizes were observed on the range of 20-50 m depth. The role as carbon and nitrogen sink of this suspension feeder has been quantified coupling distribution data with existing in situ find more studies of feeding and respiration. Along the 1.24 ha of the study area, H. papillosa yearly ingested 519.4 g C and 31.4 g N and retained 20.2 g C. As long as the physiological data are known, this new methodology could be very useful in assessing bentho-pelagic links and the capacity of being C and N sinks of a wide range of species. This new approach may be essential for the future management of benthic communities.”

system inhibitors significantly reduce the incidence of arrhythmias. However, the underlying mechanism(s) is not well understood. We aim to test the hypothesis that angiotensin II (Ang II) induces early afterdepolarizations (EADs) and triggered activities (TAs) via the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-ROS-calmodulin kinase II (CaMKII) pathway. ROS production was analyzed in the isolated rabbit myocytes loaded with ROS dye. Ang II (1-2 mu M) increased ROS fluorescence in myocytes, which was abolished by Ang II type 1 receptor blocker losartan, NADPH oxidase inhibitor apocynin, and antioxidant MnTMPyP, respectively. Action potentials were recorded using the perforated patch-clamp technique. EADs emerged in 27 out of 41 (66%) cells at 15.8 +/- 1.6 min after Ang II (1-2 mu M) perfusion. Ang II-induced EADs were eliminated by losartan, apocynin, or trolox.

Intraoperative evaluation is insensitive for the detection of sta

Intraoperative evaluation is insensitive for the detection of stage III tumors. (Surgery 2012;152:1150-7.)”
“Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and PND-1186 conformational changes. The nitrilotriacetic acid derivatives

of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback-loss of the fluorescence upon binding of the nickel ions. Herein we present the solution and solid phase synthesis of water-soluble perylene(dicarboximide) functionalized with a nitrilotriacetic acid moiety (PDI-NTA). The photophysical properties of PDI-NTA revealed an exceptional photostability and fluorescence quantum yield that remained unchanged upon addition of nickel ions. The F(1) complex of F(0)F(1)-ATP synthase from Escherichia coli, containing three hexahistidine tags, was labeled and the suitability for site-specific labeling of the new chromophore demonstrated using fluorescence correlation spectroscopy.”
“Background: Kohne et al.

[Ann Oncol 2002; 13: 308-317] showed click here that four prognostic variables can be used to classify patients with metastatic colorectal cancer (CRC) treated with 5-fluorouracil (5-FU)/leucovorin (LV) into three risk groups with different overall survival (OS). This model was applied to data from phase II/III trials of first-line bevacizumab plus 5-FU/LVwith/without irinotecan (IFL). Methods: Data on tumor sites, learn more Eastern Cooperative Oncology Group performance status, alkaline phosphatase levels and white blood cell counts were used to classify patients into Kohne prognostic high-, intermediate- and low-risk

groups. Median OS and progression-free survival (PFS) were calculated for patients receiving 5-FU/LV plus bevacizumab or placebo (n = 489) and IFL plus bevacizumab or placebo (n = 812). Results: Median OS was longer in 5-FU/LV/bevacizumab (11.2-22.6 months) than in the 5-FU/LV/placebo (5.7-17.5 months), and in the IFL/bevacizumab arm (14.3-22.5 months) than in the IFL/placebo arm (8.4-17.9 months) across the Kohne high-, intermediate- and low-risk groups. The addition of bevacizumab also extended median PFS across the Kohne risk groups compared with placebo. Conclusions: Bevacizumab improves OS and PFS across the Kohne risk classification in patients with metastatic CRC. The Kohne model can be extended to patients treated with 5-FU/LV/bevacizumab, IFL and IFL/bevacizumab and to PFS data. Copyright (c) 2008 S. Karger AG, Basel”
“Background: Many bladder pain syndrome/interstitial cystitis (BPS/IC) patients report multiple pain locations outside the pelvis.

In this study, we show that diffuse transcranial irradiation of t

In this study, we show that diffuse transcranial irradiation of the injury site with far red to near infrared (NIR) light (WARP 10 LED array, center wavelength 670 nm, irradiance 252W/m(-2), 30 min exposure), as opposed to perception

of light at this wavelength, reduced oxidative stress in areas of the ON vulnerable to secondary degeneration following partial injury. The WARP 10 NIR light treatment also prevented increases in NG-2-immunopositive oligodendrocyte precursor cells (OPCs) that occurred in ventral ON as a result of partial Autophagy Compound Library ic50 ON transection. Importantly, normal visual function was restored by NIR light treatment with the WARP 10 LED array, as assessed using optokinetic nystagmus and the Y-maze pattern discrimination task. To our knowledge, this is the first demonstration that 670-nm AC220 supplier NIR light can reduce oxidative stress and improve function in the CNS following traumatic injury in vivo.”
“To analyze the clinicopathological features and prognosis of T1mic, a, bN0M0 breast cancer.\n\nThe clinical data and survival status of 4487 cases of operable breast cancer

treated in our hospital from 2002 to 2005 were collected, including 372 cases with T1mic, a, bN0M0 breast cancers. These patients were divided into four subtypes: Luminal A, Luminal B, triple-negative and human epidermal growth factor receptor 2-positive. Disease-free survival and risk factors for recurrence were identified.\n\nWe identified 372 eligible patients. The median follow-up was 78 months (range: 5106 months). Univariate analysis showed age, adjuvant endocrine therapy, hormonal receptor and human epidermal growth factor receptor 2 were prognostic factors. Multivariate analysis Epigenetics inhibitor showed that hormonal receptor and human epidermal growth factor receptor 2 were prognostic factors. In the hormonal receptor-positive group, human epidermal growth factor receptor 2-positive patients (Luminal B) had a four times higher recurrence risk than human epidermal growth factor receptor 2 negative (Luminal

A) patients. However, there was no statistically significant difference between hormonal receptor-negative groups (triple-negative and human epidermal growth factor receptor 2-positive).\n\nHormonal receptor and human epidermal growth factor receptor 2 were independent factors of 5-year disease-free survival for patients with T1mic, a, bN0M0 breast cancer. The Luminal B group had a worse prognosis than the Luminal A group, but there was no statistically significant difference between triple-negative and human epidermal growth factor receptor 2-positive groups.”
“Background and Objectives: Our aim was to assess the efficacy and safety of infliximab (IFX) in clinical practice in three Primary Care, Hospital Centers.\n\nMaterial and Methods: From September 2004 to December 2008 62 patients (28 males, 34 females, mean age 30.

Imaging studies were not conclusive; however, brain biopsy confir

Imaging studies were not conclusive; however, brain biopsy confirmed the diagnosis. Serology was positive for anti-toxoplasma immunoglobulin G. Cerebral toxoplasmosis should be included in the differential diagnosis of patients under immunosuppressive medication who present with neurological manifestations.”
“Recent functional imaging studies demonstrated that brain exhibit coherent, synchronized activities during testing state and the dynamics may be impaired Galardin in various psychiatric illnesses. In order to investigate the change of neural dynamics in bipolar disorder, we used a new nonlinear

measurement “similarity index” to analyze the magnetoencephalography (MEG) recordings and test the hypothesis that there are synchronization changes within different frequency bands in the frontal cortex of patients with bipolar disorder. Ten

patients with bipolar I disorder during euthymic phase and ten normal controls underwent 2 min eye-closed resting recording with a whole-head 306-channel MEG system. Eleven channels of MEG data from frontal PLX3397 solubility dmso area were selected for analysis. Synchronization level in the delta (2-4 Hz), theta (4-8 Hz), alpha (8-12 Hz) and beta (12-24 Hz) bands was calculated for each subject and compared across group. The results showed that significant dynamic changes in bipolar patients can be characterized by increased synchronization of slow frequency oscillations (delta) and decreased synchronization of fast frequency oscillations (beta). Furthermore, the positive correlation between beta synchronization level and preservative errors in Wisconcin Card sorting task was found which would implicate the deficit of executive function in bipolar patients. Out findings indicate that analysis of spontaneous MEG recordings at resting state using nonlinear dynamic AZD6094 approaches may disclose the Subtle regional changes of neural dynamics in BD. (c) 2008 Elsevier Ireland Ltd. All rights reserved.”
“Background. Sentinel lymph node biopsy (SLNB) the standard at many institutions caring for noma patients. Patients with positive SLNB are

currently offered completion lymph node dissection (CLND) of the affected lymph node basin. procedure entails considerable morbidity and is often applied to patients with shortened life expectancies. Because 80% of CLNDs yield no additional positive nodes and there is no proof that CLND leads to survival improvement, criteria are needed to limit this procedure to those most likely to harbor nonsentinel lymph node (SLN) metastases.\n\nMethods. A retrospective review of 349 cases of melanoma from January 1999 to April 2007 that underwent sentinel lymph node biopsy at a single institution was done. Statistical analysis was used to compare two subgroups of patients: a positive CLND group and a negative CLND group.

“The current standard of care for head and neck cancer inc

“The current standard of care for head and neck cancer includes surgical resection of the tumor followed by targeted head and neck radiation. This radiotherapy results in a multitude of negative side effects in adjacent normal tissues. Autophagy is a cellular

mechanism that could be targeted to ameliorate these side effects based on its role in cellular homeostasis. In this study, we utilized Atg5(f/f); Aqp5-Cre mice which harbor a conditional knockout of Atg5, in salivary acinar cells. These autophagy-deficient click here mice display increased radiosensitivity. Treatment of wild-type mice with radiation did not robustly induce autophagy following radiotherapy, however, using a model of preserved salivary gland function by IGF-1-treatment prior to irradiation, we demonstrate increased autophagosome formation 6-8 hours following radiation. Additionally, administration of IGF-1 to Atg5(f/f); Aqp5-Cre mice did not preserve physiological function. Thus, autophagy appears to play a

beneficial role in salivary glands following radiation and pharmacological induction of autophagy could alleviate the negative side effects associated with therapy for head and neck cancer.”
“Two cases of a primary mediastinal ERK inhibitor manufacturer tumor showing both thymoma and seminoma elements are presented. The patients were 2 men, aged 32 and 34 years, respectively. Clinically, both patients presented with symptoms related to their mediastinal masses, namely, chest pain and shortness of breath. Neither patient had any previous history of malignancy elsewhere. Diagnostic imaging revealed the presence of large anterior mediastinal masses. Both patients underwent thoracotomy with complete resection of their tumors. Grossly, the lesions measured 6 and 8 cm in greatest diameter, respectively and were tan and firm

without areas of hemorrhage or necrosis. Both tumors appeared to be well circumscribed and grossly not involving any adjacent structures. Histologic sections showed the presence of distinct areas in the same tumor mass check details corresponding to conventional thymoma, whereas other areas corresponded to seminoma. Both components appeared to be present in almost equal proportions in the tumor. Immunohistochemical studies showed distinct labeling for each component: the thymoma component was positive for CAM5.2, Pax8, and cytokeratin 5/6; the seminoma component was positive for CAM5.2, SALL4, OCT3/4, and placental-like alkaline phosphatase. Both patients are currently alive and well 12 and 18 months after surgery, respectively. The cases herein described highlight the importance of ample sampling in mediastinal tumors and document for the first time the existence of combined thymoma thymic seminomas. (C) 2014 Elsevier Inc. All rights reserved.

The feasibility of using F-18-SKI696 as a PET agent was examined

The feasibility of using F-18-SKI696 as a PET agent was examined in vivo, and F-18-SKI696 PET was shown to visualize K562 tumor xenografts in nude mice. The tumor was visible on PET 1 h after injection, with uptake of 1% of the injected dose. Biodistribution studies IPI-145 cost in K562-bearing mice were also performed, and the uptake of F-18-SKI696 (percentage injected dose per gram) for each organ was calculated. Conclusion: The results of the binding assay

showed that SKI696 has selectivity and binding affinity comparable to imatinib. Small-animal PET of K562 tumor-bearing mice using F-18-SKI696 as a PET agent displayed promising tumor uptake and tumor-to-nontarget contrast. Because F-18-SKI696 has been taken up in vivo by tumors that overexpress Bcr-Abl, we are exploring a possible role for identifying

tumors that will respond to imatinib before therapy.”
“Plant biomass is a renewable and potentially sustainable resource for the production of liquid biofuels and a multitude of bio-based materials. To tailor plants for biofuel production, a powerful gene discovery program targeted to cell ACY-738 wall recalcitrance genes is needed. In parallel, a system is required that reveals the pleiotropic effects of gene modifications and that delivers the fundamental knowledge necessary for successful gene stacking. In our opinion, these objectives can be pioneered through a systems biology approach in Arabidopsis. We develop our ideas with a focus on the lignin biosynthetic pathway, because lignin is among the most important factors determining cell wall recalcitrance.”
“Background: Human T-cell leukemia virus type-1 (HTLV-1) is the causative retrovirus of adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1 gene expression is maintained at low levels in vivo by unknown mechanisms. A combination therapy of interferon-alpha (IFN-alpha) and zidovudin (AZT) shows therapeutic effects in ATL patients, although

its mechanism is also obscure. We previously found that viral gene expression in IL-2-dependent HTLV-1-infected T-cells (ILTs) derived from ATL patients was markedly suppressed by stromal cells through a type I IFN response. Here, we investigated the effects of IFN-alpha with or without AZT on viral gene expression and cell growth in ILTs.\n\nResults: ILTs expressed variable but lower amounts of HTLV-1 Tax protein than HTLV-1-transformed HUT102 cells. Following the addition of IFN-alpha, the amounts of HTLV-1 p19 in the supernatants of these cells decreased in three days, while HTLV-1 gene expression decreased only in ILTs but not HUT102 cells. IFN-alpha also suppressed the spontaneous HTLV-1 induction in primary ATL cells cultured for 24 h. A time course study using ILTs revealed that the levels of intracellular Tax proteins decreased in the first 24 h after addition of IFN-alpha, before the reduction in HTLV-1 mRNA levels.